The Use of Monoclonal Antibodies to Study the Structure and Function of Photosystem II

使用单克隆抗体研究光系统II的结构和功能

• 批准号: 9118333
• 负责人: Steven Berg
• 金额: $ 18万
• 依托单位: Winona State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-05-01 至 1995-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9118333&HistoricalAwards=false
• 关键词: Use; Monoclonal; Antibodies; Study; Structure

The laboratory has been using monoclonal antibodies (MABs) to study the topology of selected proteins associated with photosynthesis in higher plants. This is done by isolating and cloning hybridomas which secrete MAbs of interest. Once obtained, the ability of the Mabs to bind to various preparations (rightsideout thylakoids, insideout thylakoids, isolated Photosystem II membranes, isolated proteins, etc.) can be asessed and in so doing it is possible to determine the exposure of the specific epitopes to which the MAbs bind. By cross correlating our Mab binding data and epitope determination with hypothetical models of protein topography based on hydropathic analyseSs of protein primary structure, more topologically correct models of photosynthetic protein complexes of the thylakoid membrane can be developed.

该实验室一直在使用单克隆抗体（MAB）， 研究所选蛋白质的拓扑结构， 高等植物的光合作用 这是通过隔离和 克隆分泌目的MAb的杂交瘤。 一旦 获得，单克隆抗体结合各种抗体的能力 制备物（右侧向外类囊体，内侧向外类囊体， 分离的光系统II膜、分离的蛋白质等）可以 这样做，就有可能确定暴露于 单克隆抗体结合的特异性表位。 通过互相关 我们的Mab结合数据和表位测定， 基于亲水分析的蛋白质拓扑结构模型 蛋白质一级结构，拓扑正确的模型， 类囊体膜的光合蛋白复合物可以 开发