NMR Studies of Protein - Nucleic Acid Interactions

蛋白质-核酸相互作用的 NMR 研究

• 批准号: 9118369
• 负责人: Stephen White
• 金额: $ 33万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-05-01 至 1995-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9118369&HistoricalAwards=false
• 关键词: NMR; Studies; Protein; Nucleic; Acid

A structural investigation by Nuclear Magnetic Resonance (NMR) spectroscopy of RNA-protein interactions will be carried out. These interactions are poorly understood, and their importance in areas such as RNA processing and RNA viruses is rapidly becoming appreciated. The systems to be studied include the prokaryotic ribosome, the eukaryotic spliceosome and the regulation of transcription in bacteriophage T4. In the ribosome and spliceosome, specific RNA-protein interactions are crucial to structure and function, and components of each including individual proteins, RNA fragments and complexes will be isolated and studied. In addition, the structure of the putative DNA- binding domain of a vital protein that controls transcription in bacteriophage T4 will be determined. %%% Interactions between proteins and nucleic acids are central to many cellular processes and the results of this research will provide important insights into the mechanisms by which proteins recognize specific nucleic acid sequences.

利用核磁共振（NMR）光谱对rna -蛋白质相互作用进行结构研究。人们对这些相互作用知之甚少，但它们在RNA加工和RNA病毒等领域的重要性正迅速得到认识。拟研究的系统包括原核核糖体、真核剪接体和噬菌体T4的转录调控。在核糖体和剪接体中，特定的RNA-蛋白质相互作用对其结构和功能至关重要，将分离和研究它们的组成部分，包括单个蛋白质、RNA片段和复合物。此外，将确定控制T4噬菌体转录的重要蛋白质的推定DNA结合域的结构。蛋白质和核酸之间的相互作用是许多细胞过程的核心，这项研究的结果将为蛋白质识别特定核酸序列的机制提供重要的见解。