NMR Studies of Protein - Nucleic Acid Interactions

核磁共振（NMR）结构研究 将进行RNA-蛋白质相互作用的光谱分析。 人们对这些相互作用知之甚少， RNA加工和RNA病毒等领域正在迅速成为 赞赏. 待研究的系统包括原核生物 核糖体，真核剪接体和调控 在噬菌体T4中的转录。 在核糖体中， 在剪接体中，特异性RNA-蛋白质相互作用对于 结构和功能，以及每个组件，包括 将分离单个蛋白质、RNA片段和复合物 和研究 此外，假定的DNA结构- 一种重要蛋白质的结合区，控制转录， 将测定噬菌体T4。 %%% 蛋白质和核酸之间的相互作用是 许多细胞过程和这项研究的结果将 提供了重要的见解的机制， 识别特定的核酸序列。

A structural investigation by Nuclear Magnetic Resonance (NMR) spectroscopy of RNA-protein interactions will be carried out. These interactions are poorly understood, and their importance in areas such as RNA processing and RNA viruses is rapidly becoming appreciated. The systems to be studied include the prokaryotic ribosome, the eukaryotic spliceosome and the regulation of transcription in bacteriophage T4. In the ribosome and spliceosome, specific RNA-protein interactions are crucial to structure and function, and components of each including individual proteins, RNA fragments and complexes will be isolated and studied. In addition, the structure of the putative DNA- binding domain of a vital protein that controls transcription in bacteriophage T4 will be determined. %%% Interactions between proteins and nucleic acids are central to many cellular processes and the results of this research will provide important insights into the mechanisms by which proteins recognize specific nucleic acid sequences.