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Regulation of Protein Synthesis in Developing Oat Seeds

燕麦种子发育过程中蛋白质合成的调控

基本信息

批准号：
9118656
负责人：
Brian Larkins
金额：
$ 15万
依托单位：
University of Arizona
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1992
资助国家：
美国
起止时间：
1992-03-01 至 1994-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9118656&HistoricalAwards=false
关键词：
Regulation Protein Synthesis Developing Oat

项目摘要

Genes for the oat storage proteins globulin and avenin are expressed in a differential manner during seed formation. The cause for this differential expression has been studied by Dr. Larkins as a component of his research on the biochemistry of seed storage proteins. Preliminary evidence has narrowed the control of expression to the translation step of the process. The investigators propose to explore the molecular basis for the differential synthesis of storage proteins in the oat system. They have constructed a series of transcription vectors encoding avenins and globulins and will analyze the translation of synthetic mRNAs in both wheat germ extracts and xenopus oocytes. This analysis may allow them to identify structural features of the mRNAs that cause the preferential translation of the globulin transcripts. They plan to prepare a tRNA-dependent in vitro translation system and assess the role of the tRNA population of oat endosperm in storage protein synthesis. This will allow them to determine if translation termination or protein transport play a critical role in regulating storage protein accumulation. A thorough knowledge of how storage protein synthesis is controlled will give us insight into the mechanisms regulating the growth and development of seeds and provide a basis for future improvements in the nutritional quality of oats and other cereals by genetic engineering.

燕麦贮藏蛋白球蛋白和燕麦蛋白的基因是在种子形成期间以差异方式表达。这种差异表达的原因已被研究，拉金斯博士作为他的生物化学研究的一部分种子贮藏蛋白初步证据显示控制表达到该过程的翻译步骤。研究人员建议探索这种现象的分子基础。燕麦系统中贮藏蛋白的差异合成。他们构建了一系列编码燕麦蛋白的转录载体和球蛋白，并将分析合成mRNA的翻译在小麦胚芽提取物和爪蟾卵母细胞中。该分析可以让他们识别mRNA的结构特征，导致球蛋白转录本的优先翻译。他们计划制备一种依赖于tRNA的体外翻译系统并评估燕麦胚乳tRNA群体在贮藏蛋白合成这将使他们能够确定，翻译终止或蛋白质转运起关键作用在调节贮藏蛋白质积累方面。有透彻的了解储存蛋白合成是如何控制的调节种子生长发育的机制并为未来改善营养状况提供基础。通过基因工程提高燕麦和其他谷物的质量。