The Role of Mago Nashi in Drosophila Development

Mago Nashi 在果蝇发育中的作用

• 批准号: 9119535
• 负责人: Robert Boswell
• 金额: $ 31万
• 依托单位: University of Colorado at Boulder
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-01-01 至 1996-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9119535&HistoricalAwards=false
• 关键词: Role; Mago; Nashi; Drosophila; Development

In many organisms early determinative events are established by the differential distribution of informational molecules(cytoplasmic determinants). In Drosophila,the distribution of these determinants is established by genes that make a strictly maternal contribution to the developing embryo. Drosophila serves as a model system for studying genes that produce these determinants and/or are involved in properly distributing these determinants within the embryo. The PI is studying Mango Nashi a maternal effect gene required both for the determination of the primordial germ cells and establishment of the anteroposterior axis of the embryo. The mago locus is novel in that mutations of the locus disrupt germ cell formation and are also capable of causing a replacement of anterior embryonic structures by posterior tail and abdominal structures. Double mutants between mago and alleles of loci crucial for delineation of the anteroposterior axis of the embryo will be used to better define the role of mago in specifying the anteroposterior axis of the embryo. Genetic isolation of new mago alleles and developmental analysis of thes alleles will provide information necessary for refining models dipicting the role of mago+ in early embryonic development. A molecular characterization of the mago locus will include: (a) identification of mago mRNA(s), and isolation and sequencing of cDNA clones representative of mago mRNA; and (b) determination of the distribution of the mago transcript(s) and protein(s) in oocytes and embryos. This combined analysis should significantly improve our understanding of mago+ function in early Drosophila development. Thus, these studies should provide insights into the mechanisms underlying germ cell formation and the establishment of positional information in the early embryo.

在许多生物体中，早期决定性事件是由信息分子（细胞质决定因素）的差异分布确定的。在果蝇中，这些决定因素的分布是由基因决定的，这些基因对发育中的胚胎有严格的母系贡献。果蝇是研究产生这些决定因素和/或参与在胚胎中正确分配这些决定因素的基因的模型系统。该项目正在研究芒果纳西，这是一种母体效应基因，对确定原始生殖细胞和建立胚胎的前后轴都是必需的。马戈基因座的新颖之处在于，基因座的突变会破坏生殖细胞的形成，并且还能够导致胚胎前部结构被后尾和腹部结构所取代。mago与胚胎前后轴定位关键位点等位基因之间的双突变将被用来更好地定义mago在胚胎前后轴定位中的作用。新的mago等位基因的遗传分离和这些等位基因的发育分析将为完善描述mago+在早期胚胎发育中的作用的模型提供必要的信息。mago基因座的分子鉴定将包括：(A) mago mRNA的鉴定，以及代表mago mRNA的cDNA克隆的分离和测序；(b)测定mago转录物和蛋白在卵母细胞和胚胎中的分布。这一综合分析将显著提高我们对果蝇早期发育中mago+功能的理解。因此，这些研究应该为生殖细胞形成的机制和早期胚胎中位置信息的建立提供见解。