The Mechanism of Distribution of Tudor Transcript and Protein in Oogenesis and Embryogenesis

本研究的目的是描述 tudor，一个黑腹果蝇母体效应位点，在两个 早期胚胎发生、生殖细胞决定的中心事件 和腹部分割。 设计了实验方法 来确定指定空间和时间 卵子发生过程中都铎产物的分布模式， 胚胎发生 这将涉及：1）比较tudor mRNA 和蛋白质分布在生殖细胞形成和前/ 野生型中轴的建立和母体效应 突变胚胎; 2）RNA和/或蛋白质的测定 负责都铎王朝产物定位于 胚胎后极;和3）确定是否tudor 在晚期胚胎发生中需要表达。 实验 目标将通过介绍和表达 在转基因动物中的修饰的Tudor基因构建体。 这些 研究预计将有助于普遍了解 胚胎细胞命运决定的分子机制。

The objective of this research is to characterize the role of tudor, a Drosophila melanogaster maternal effect locus, in two central events of early embryogenesis, germ cell determination and abdomen segmentation. The experimental approach is designed to determine the mechanisms that specify the spatial and temporal patterns of tudor product distribution during oogenesis and embryogenesis. This will involve: 1) comparison of tudor mRNA and protein distribution during germ cell formation and anterior/ posterior axis establishment in wild type and maternal effect mutant embryos; 2) determination of the RNA and/or protein sequences responsible for localization of tudor products to the embryonic posterior pole; and 3) determination whether tudor expression is required in late embryogenesis. Experimental objectives will be carried out by introduction and expression of modified tudor gene constructs in transgenic animals. These studies are expected to contribute to general understanding of molecular mechanism(s) of embryonic cell fate determination.