Generation and Utilization of Monoclonal Antibodies as Probes of Slow NGF Receptor Structure and Function

作为慢 NGF 受体结构和功能探针的单克隆抗体的产生和利用

• 批准号: 9120836
• 负责人: Stuart Feinstein
• 金额: $ 18.92万
• 依托单位: University of California-Santa Barbara
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-03-15 至 1996-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9120836&HistoricalAwards=false
• 关键词: Generation; Utilization; Monoclonal; Antibodies; Probes

Intercellular biochemical communication is a ubiquitous feature in biology serving to mediate an enormous range of regulatory functions. In vertebrate nervous systems, targets of innervation secrete proteins known as neurotrophic factors, which are subsequently received by the innervating neuron. Acquisition of sufficient neurotrophic factor by individual innervating neurons is essential for the proper development and maintenance of the nervous system. Interruption of the flow of neurotrophic factor from the target to the neurons result in the rapid neuronal destruction of the affected region. In Dr. Feinstein's project, he will examine the molecular mechanisms by which Nerve Growth Factor (NGF), the best understood among the neurotrophic factors, mediates its action upon responsive neuronal cells. NGF action is known to be initiated by the interaction of NGF with "slow" (also known as "high affinity") NGF receptors located in the neuronal plasma membrane. He will first utilize a variety of immunological strategies to generate novel molecular probes. Then, he will use these probes as tools to investigate the molecular composition of the slow NGF receptors as well as the functions mediated by the slow NGF receptors. These studies should markedly contribute to our understanding of neurotrophic factor action and the development and maintenance of the nervous system.*** //

细胞间的生化通讯是一个普遍存在的特征， 生物学起着调节各种调节的作用， 功能协调发展的 在脊椎动物神经系统中，神经支配的目标 分泌称为神经营养因子的蛋白质， 随后由神经支配的神经元接收。 收购 充足的神经营养因子通过个别支配神经元 对适当发展和维护 神经系统 神经营养因子流中断 从目标到神经元的快速神经元 破坏受影响的地区。 在范斯坦博士的项目中， 他将研究神经生长的分子机制 神经生长因子（NGF）是神经营养因子中最好的， 介导其对响应神经元细胞的作用。 NGF的作用是 已知是由NGF与“慢”（也称为“慢”）的相互作用引发的 称为“高亲和力”）位于神经元中的NGF受体 质膜 他将首先利用各种免疫学 产生新型分子探针的策略。 然后，他将使用 这些探针作为工具来研究的分子组成， 缓慢的NGF受体以及由 减缓神经生长因子受体。 这些研究应该有助于 我们对神经营养因子作用的理解和发展 和神经系统的维护。* //