Birth Order and Cell Fate in an Embryonic Neuronal Lineage

胚胎神经元谱系的出生顺序和细胞命运

• 批准号: 9120863
• 负责人: Melody V. S. Siegler
• 金额: $ 19.56万
• 依托单位: Emory University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-03-15 至 1995-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9120863&HistoricalAwards=false
• 关键词: Birth; Order; Cell; Fate; Embryonic

The nervous system contains a stereotyped complement of neurons with different properties. Neurons have different shapes, respond to and release different biochemical messengers, and exert their effects on unique subsets of target neurons. The processes underlying the development of this neuronal specificity will be investigated in the simple nervous system of the grasshopper. The investigator has previously characterized a group of 100 midline neurons that arise from a single identified precursor stem cell. This group of neurons contains three basic cell types that function in different sets of behaviors. It will be determined how the final complement of neurons in the group arises temporally during development, over what time course neurons are added to the population and when and to what extent cell death reduces the population to its final size. In addition, the birthdays of identified neurons, i.e. the timing of a neuron's final cell division, will be correlated with cell lineage to test the hypothesis that birth order and cell type are related, with sequential switches occurring during development to produce efferent neurons, local interneurons, and then intersegmental interneurons. Differential staining of cell types and birthdating of individual neurons by labeling DNA during cell divisions will be applied. The system is unique in the high degree of resolution for identifying individual neurons of a population and the potential for spanning several levels of inquiry in studying the relationships between developmental events and the functioning of mature neuronal circuits. The study of neurogenesis in this simple nervous system that is easily accessible to experimental manipulation promises to increase our general knowledge of processes involved in the formation of different kinds on neurons.

神经系统包含一个固定的神经元 不同的属性。 神经元有不同的形状， 释放不同的生化信使，并发挥它们的作用。 对靶神经元的独特子集的影响。 的过程 这种神经元特异性发展的基础将是 在蚱蜢的简单神经系统中进行了研究。 的 研究者先前描述了一组100例中线 神经元是由一个单一的前体干细胞产生的。 这组神经元包含三种基本的细胞类型， in different不同sets组of behaviors行为.将决定最终如何 组中的神经元的补充在时间上出现， 发展，在什么时间过程中神经元被添加到 以及细胞死亡何时以及在多大程度上减少了 人口达到最终规模。 此外， 识别出的神经元，即神经元的最后一个细胞的时间 分裂，将与细胞谱系相关，以测试 假设出生顺序和细胞类型相关， 在开发过程中发生的顺序转换， 传出神经元，局部中间神经元，然后节间 中间神经元 细胞类型的差异染色和出生日期 通过在细胞分裂过程中标记DNA， 应用。 该系统的独特之处在于高分辨率， 识别群体中的单个神经元， 跨越几个层次的研究， 发展事件和功能之间的关系 成熟的神经回路 在这个简单的神经发生的研究 神经系统，很容易接近实验 操纵承诺增加我们的一般知识 参与不同种类神经元形成的过程。