Interrogating protein hydration by terahertz time-domain spectroscopy

通过太赫兹时域光谱研究蛋白质水合

• 批准号: LP160100325
• 负责人: Prof Vincent Wallace
• 金额: $ 18.74万
• 依托单位: The University of Western Australia
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2016
• 资助国家: 澳大利亚
• 起止时间: 2016-11-09 至 2020-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP160100325
• 关键词: Interrogating; protein; hydration; terahertz; time

Interrogating protein hydration by terahertz time-domain spectroscopy. This project aims to develop advanced terahertz time-domain spectroscopy (THz-TDS) techniques to understand how a protein’s interaction with excipients in solution can alter the hydration layers around them and how this affects properties such as viscosity and stability. THz-TDS can detect overlapping extended hydration layers around proteins, revealing new knowledge in protein-protein interactions and protein behaviour in an aqueous environment. The intended outcome is to use THz-TDS to better understand how proteins such as monoclonal antibodies interact in high concentration solutions, and in doing so make better medicines.

太赫兹时域光谱法研究蛋白质水合作用。该项目旨在开发先进的太赫兹时域光谱（THz-TDS）技术，以了解蛋白质与溶液中赋形剂的相互作用如何改变它们周围的水合层，以及这如何影响粘度和稳定性等特性。THz-TDS可以检测蛋白质周围重叠的扩展水化层，揭示蛋白质-蛋白质相互作用和蛋白质在水环境中行为的新知识。预期的结果是使用THz-TDS来更好地了解蛋白质（如单克隆抗体）如何在高浓度溶液中相互作用，从而制造出更好的药物。