Development of Ultra-sensitive Immunoassay Techniques and Immunosensors Based on Enzyme Amplification and the Avidin-Biotin Interaction

基于酶扩增和亲和素-生物素相互作用的超灵敏免疫测定技术和免疫传感器的发展

• 批准号: 9123718
• 负责人: Moore Asouzu
• 金额: $ 11万
• 依托单位: Troy University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-05-01 至 1995-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9123718&HistoricalAwards=false
• 关键词: Development; Ultra; sensitive; Immunoassay; Techniques

This Research in Undergraduate Institutions (RUI) project is in the general area of analytical and surface chemistry and in the subfield of bioanalysis. It is jointly supported by the Division of Chemistry and the Experimental Program to Stimulate Competitive Research (EPSCoR) Office. During the tenure of this three-year continuing grant, Professor Asouzu and his students will develop and study enzyme immunoassay methods and immunosensors. The incorporation of avidin, streptavidin, biotin, and their enzyme and antibody conjugates into immunoassays and immunosensor design will be evaluated. Enzymes and antibodies will be used in both their solubilized and immobilized forms. Enzyme immunoassay techniques will be combined with flow injection analysis and amperometric detection to develop a multi-antigen immunoassay method. %%% This project includes a well-conceived, multi-step paradigm for introducing undergraduate students, including freshmen, to applications of enzymes and enzyme kinetic methods for bioanalysis. The research is expected not only to lead to new developments in this rapidly expanding field, but also to contribute strongly to the research and training infrastructure of Troy State University and enable Professor Asouzu, a minority investigator, to initiate a competitive research program.

该本科机构研究（RUI）项目属于分析和表面化学的一般领域以及生物分析的子领域。 该项目由化学系和刺激竞争性研究实验计划（EPSCoR）办公室共同支持。 在这项为期三年的持续资助期间，Asouzu教授和他的学生将开发和研究酶免疫分析方法和免疫传感器。 将评估抗生物素蛋白、链霉抗生物素蛋白、生物素及其酶和抗体缀合物纳入免疫测定和免疫传感器设计的情况。 酶和抗体将以溶解和固定形式使用。 酶免疫分析技术将与流动注射分析和电流检测相结合，开发多抗原免疫分析方法。 %%% 该项目包括一个构思良好的多步骤范例，用于向本科生（包括新生）介绍酶和酶动力学方法在生物分析中的应用。 这项研究不仅有望在这个快速扩展的领域带来新的发展，而且还将为特洛伊州立大学的研究和培训基础设施做出巨大贡献，并使少数族裔研究员 Asouzu 教授能够启动一项有竞争力的研究计划。