Development of Ultra-sensitive Immunoassay Techniques and Immunosensors Based on Enzyme Amplification and the Avidin-Biotin Interaction

这个本科院校的研究项目是在分析和表面化学的一般领域，以及生物分析的子领域。它由化学部和刺激竞争研究的实验计划(EPSCoR)办公室联合支持。在这项为期三年的持续资助期间，Asouzu教授和他的学生将开发和研究酶免疫分析方法和免疫传感器。将对亲和素、链霉亲和素、生物素及其酶和抗体结合物在免疫分析和免疫传感器设计中的应用进行评估。酶和抗体将以其溶解和固定的形式使用。酶免疫分析技术将与流动注射分析和安培检测相结合，开发一种多抗原免疫分析方法。这个项目包括一个构思良好的、多步骤的范例，向包括新生在内的本科生介绍酶和酶动力学方法在生物分析中的应用。这项研究不仅有望在这一迅速扩大的领域带来新的发展，还将为特洛伊州立大学的研究和培训基础设施做出巨大贡献，并使少数族裔研究人员Asouzu教授能够启动一个具有竞争力的研究项目。

This Research in Undergraduate Institutions (RUI) project is in the general area of analytical and surface chemistry and in the subfield of bioanalysis. It is jointly supported by the Division of Chemistry and the Experimental Program to Stimulate Competitive Research (EPSCoR) Office. During the tenure of this three-year continuing grant, Professor Asouzu and his students will develop and study enzyme immunoassay methods and immunosensors. The incorporation of avidin, streptavidin, biotin, and their enzyme and antibody conjugates into immunoassays and immunosensor design will be evaluated. Enzymes and antibodies will be used in both their solubilized and immobilized forms. Enzyme immunoassay techniques will be combined with flow injection analysis and amperometric detection to develop a multi-antigen immunoassay method. %%% This project includes a well-conceived, multi-step paradigm for introducing undergraduate students, including freshmen, to applications of enzymes and enzyme kinetic methods for bioanalysis. The research is expected not only to lead to new developments in this rapidly expanding field, but also to contribute strongly to the research and training infrastructure of Troy State University and enable Professor Asouzu, a minority investigator, to initiate a competitive research program.