Digital Imaging Microscope

该建议要求支持多学科 努力开发数字成像显微镜， 分子分布变化的分析和控制 潜在的细胞功能。 我们建议加强空间 和荧光成像的时间分辨率， 开发新的显微镜和照相机系统， 毫秒域，并通过开发计算 进一步提高分辨率的方法。 我们将开发 二维、三维、三维定量分析方法 时间序列荧光图像和用于确定误差 给定分子浓度估计值的界限 在一个隔间里 我们将提供强大的能力， 利用集成工具进行图像的测量和分析 用于图像显示和与自动化功能的交互 提取算法 我们将开发工具来调查 两个或多个分子之间的关系， 彼此 我们将增加算法的计算量 速度，使数字成像显微镜成为一个真实的- 计时器：一种允许交互式或自动控制的计时装置 实验 最后，我们将向仪器添加 控制和改变分子分布的能力， 活细胞的控制光解的“笼”化合物。

This proposal requests support for a multidisciplinary effort to develop the digital imaging microscope for the anlaysis and control of changes in molecular distribution underlying cell function. We propose to enhance the spatial and temporal resolution of fluorescence imaging by developing new microscope and camera systems for imaging in the millisecond domain and by developing computational methods for further extending resolution. We will develop methodology for quantitative analysis of 2-D, 3-D and 3-D time series fluorescence images and for determining error bounds on estimates of the concentration of a given molecule in a compartment. We will provide a powerful capability for the measurement and analysis of images by integrating tools for image display and interaction with automated feature extraction algorithms. We will develop tools to investigate the relationship of two or more molecules with respect to one another. We will incrased the algorithm computation speed to make the digital imaging microscope into a real- time device that allows interactive or automated control of experiments. Finally, we will add to the instrument the ability to control and modify molecular distribution in living cells by controlled photolysis of "caged" compunds.