Long and Medium-Term Research: Cellular Analysis of Cell Cycle Regulatory Phosphoproteins and Cytoskeletal Proteins in Developmental Mutants of Nicotiana and Arabidopsis

长期和中期研究：烟草和拟南芥发育突变体中细胞周期调节磷蛋白和细胞骨架蛋白的细胞分析该奖项由外国卓越中心长期和中期研究计划颁发，这使得美国科学家和工程师能够在国外被证明是卓越的研究中心进行为期三到十二个月的研究。该项目的奖项提供了联合研究的机会，并提供了使用国外独特或互补的设施、专业知识和实验条件的机会。该奖项将支持海迪·S·费勒博士为期12个月的博士后研究访问，与简·A·特拉斯博士在法国凡尔赛的国家农业研究所合作。费勒博士计划确定几个烟草和拟南芥发育突变体的特征，这些突变体在细胞周期调节和分裂平面排列方面受到干扰。利用原位细胞生物学技术，她将研究一组与细胞周期控制有关的高度保守的磷酸蛋白和细胞骨架蛋白的表达和亚细胞定位。据推测，这些蛋白质的一个子集将作为区分正常和异常细胞周期控制的标记，这样的比较将有助于确定细胞周期调节对植物发育的贡献。分析cdc2和mpm-2磷酸化蛋白调节子的细胞和亚细胞定位模式以及潜在的细胞骨架原位靶标将更好地确定它们在体内的相互作用功能。像这样的实验将需要最终了解细胞周期调节，因为它发生在植物中。该奖励建议提供国际旅行经费和12个月的津贴。

Long and Medium-Term Research: Cellular Analysis of Cell Cycle Regulatory Phosphoproteins and Cytoskeletal Proteins in Developmental Mutants of Nicotiana and Arabidopsis This award is under the Long and Medium-Term Research at Foreign Centers of Excellence Program, which enables U.S. scientists and engineers to conduct three to twelve months of research abroad at research centers of proven excellence. The program's awards provide opportunities for joint research, and the use of unique or complementary facilities, expertise and experimental conditions abroad. This award will support a twelve-month postdoctoral research visit by Dr. Heidi S. Feiler to work with Dr. Jan A. Traas at the Institut National de la Recherche Agronomique in Versailles, France. Dr. Feiler plans to characterize several Nicotiana and Arabidopsis developmental mutants, which are perturbed in cell cycle regulation and division plane alignment. Using in situ cell biology techniques, she will study the expression and subcellular localization of a set of highly conserved phosphoproteins and cytoskeletal proteins implicated in cell cycle control. It is presumed that a subset of these proteins will act as markers delineating between normal and aberrant cell cycle control and that comparisons such as these will help identify the contribution of cell cycle regulation to plant development. The analysis of cellular and subcellular localization patterns of the cdc2 and MPM-2 phosphoprotein regulators and potential cytoskeletal targets in situ will better define their interactive, in vivo functions. Experiments such as these will be required to ultimately understand cell cycle regulation as it occurs within the plant. The award recommendation provides funds to cover international travel and a stipend for twelve months.