Long and Medium-Term Research: Cellular Analysis of Cell Cycle Regulatory Phosphoproteins and Cytoskeletal Proteins in Developmental Mutants of Nicotiana and Arabidopsis

中长期研究：烟草和拟南芥发育突变体中细胞周期调节磷酸化蛋白和细胞骨架蛋白的细胞分析本奖项属于国外卓越中心长期和中期研究项目。它使美国科学家和工程师能够在国外公认的卓越研究中心进行三到十二个月的研究。该计划的奖励为联合研究提供了机会，并利用独特或互补的设施、专业知识和国外的实验条件。该奖项将支持Heidi S. Feiler博士与Jan a . Traas博士在法国凡尔赛国家农学研究所进行为期12个月的博士后研究访问。Feiler博士计划描述几种烟草和拟南芥发育突变体，这些突变体在细胞周期调节和分裂平面排列中受到干扰。利用原位细胞生物学技术，她将研究与细胞周期控制有关的一组高度保守的磷酸化蛋白和细胞骨架蛋白的表达和亚细胞定位。据推测，这些蛋白质的一个子集将作为描述正常和异常细胞周期控制之间的标记，并且诸如此类的比较将有助于确定细胞周期调节对植物发育的贡献。对cdc2和MPM-2磷酸化蛋白调控因子和潜在细胞骨架靶点的细胞和亚细胞定位模式的分析将更好地确定它们在体内的相互作用功能。这样的实验将需要最终理解细胞周期调节，因为它发生在植物内。该奖项建议提供资金用于支付国际旅行和12个月的津贴。

Long and Medium-Term Research: Cellular Analysis of Cell Cycle Regulatory Phosphoproteins and Cytoskeletal Proteins in Developmental Mutants of Nicotiana and Arabidopsis This award is under the Long and Medium-Term Research at Foreign Centers of Excellence Program, which enables U.S. scientists and engineers to conduct three to twelve months of research abroad at research centers of proven excellence. The program's awards provide opportunities for joint research, and the use of unique or complementary facilities, expertise and experimental conditions abroad. This award will support a twelve-month postdoctoral research visit by Dr. Heidi S. Feiler to work with Dr. Jan A. Traas at the Institut National de la Recherche Agronomique in Versailles, France. Dr. Feiler plans to characterize several Nicotiana and Arabidopsis developmental mutants, which are perturbed in cell cycle regulation and division plane alignment. Using in situ cell biology techniques, she will study the expression and subcellular localization of a set of highly conserved phosphoproteins and cytoskeletal proteins implicated in cell cycle control. It is presumed that a subset of these proteins will act as markers delineating between normal and aberrant cell cycle control and that comparisons such as these will help identify the contribution of cell cycle regulation to plant development. The analysis of cellular and subcellular localization patterns of the cdc2 and MPM-2 phosphoprotein regulators and potential cytoskeletal targets in situ will better define their interactive, in vivo functions. Experiments such as these will be required to ultimately understand cell cycle regulation as it occurs within the plant. The award recommendation provides funds to cover international travel and a stipend for twelve months.