Modulation of active oxygen during pathogenesis of Pseudomonas syringae.

丁香假单胞菌发病过程中活性氧的调节。

• 批准号: 9203188
• 负责人: Martin Klotz
• 金额: $ 13.51万
• 依托单位: Utah State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-03-01 至 1995-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9203188&HistoricalAwards=false
• 关键词: Modulation; active; oxygen; during; pathogenesis

Successful colonization of plant tissue by Pseudomonas syringae appears to require the ability to cope with oxidative stree caused by the HOOH produced during early phases of the interaction. P. syringae strains were found to contain 10 to 100-fold higher levels of catalase activity than their nonpathogenic relatives, P. fluorescens and P. putida. At least 8 catalase isozymes were detected in lysates of P. syringae pv. glycinea race 4. Two of these isozymes were unique to the periplasm. Catalase activity in P. syringae strains was dependent on the growth phase of the culture. The specific activity of cytoplasmic catalases increased during the transition to stationary phase. These results are consistent with the involvement of multiple catalase isozymes in the reduction of oxidative stress during pathogenesis by these bacteria.

假单胞菌在植物组织中的成功定殖 似乎需要有能力科普氧化应激引起的 在相互作用的早期阶段产生的HOOH。 P. 在所有菌株中， 过氧化氢酶活性显著高于非致病性近缘种; 荧光假单胞菌和恶臭假单胞菌。 至少有8种过氧化氢酶同工酶 在P.大豆小种4. 两 这些同工酶是周质所特有的。 过氧化氢酶活性 P. pastingae菌株依赖于生长阶段的 文化 细胞质过氧化氢酶比活性升高 在过渡到稳定阶段。 这些结果 与多种过氧化氢酶同工酶的参与一致， 在发病过程中氧化应激的减少 细菌