The Movement and Geographic Origin of Marine Larvae: A Molecular and Genetic Approach

为了了解招募在人口中的作用 海洋物种的动态，有高幼虫扩散，这是 为了了解幼虫的运动， 地理起源和控制他们当地的力量 丰饶。 跟踪幼虫运动的方法已经 局限于短时间尺度或封闭系统内， 在高分散体系中的应用价值有限 因为幼虫复苏的困难。 大蚌 紫贻贝由一个复杂的兄弟物种组成， 在亲本形式之间的离散接触区域中杂交。 个体贻贝幼虫可以被遗传鉴定为具有 来自父母群体，或来自 包含杂交种的种群。杂交幼虫只能有 起源于包含双亲类群或F1的群体 混血儿使用该系统，可以获得 在几个地理尺度上的幼虫起源的信息。 需要新的技术来从基因上识别个体 幼虫 本研究将开发的方法 程序将允许直接测定单个幼虫， 最少的准备和高的处理速率。 一旦 技术已经开发出来，将有可能确定 散布或补充幼虫的地理起源。一旦制订 这项新技术将被用来研究幼虫的传播， 贻贝种群之间的接触区。

In order to understand the role of recruitment in the population dynamics of marine species which have high larval dispersal it is necessary to understand the movement of the larvae, their geographic origin and the forces that control their local abundance. Methods for tracking larval movements have been restricted to short time scales or within closed systems but their application to high dispersal systems has been of limited value because of the difficulties with larval recovery. The mussel Mytilus edulis consists of a complex of sibling species that hybridize in discrete areas of contact between the parental forms. Individual mussel larvae can be genetically identified as having originated from either of the parental population, or from populations that contain hybrids. Hybrid larvae can only have originated from populations that contained both parent taxa or F1 hybrids. Using this system it will be possible to obtain information on the origin of larvae at several geographic scales. New techniques are required to genetically identify individual larvae. The methods which will be developed in this research program will allow individual larvae to be assayed directly, with minimal preparation and a high rate of processing. Once the techniques have been developed it will be possible to identify the geographic origin of dispersing or recruited larvae. Once developed this new technique will be employed to study larval dispersal in regions of contact between Mytilus populations.