Regulation of Yeast Phosphatidate Phosphatase

酵母磷脂酸磷酸酶的调节

• 批准号: 9204588
• 负责人: George Carman
• 金额: $ 23万
• 依托单位: Rutgers University New Brunswick
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-15 至 1996-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9204588&HistoricalAwards=false
• 关键词: Regulation; Yeast; Phosphatidate; Phosphatase

Phosphatidate (PA) phosphatase (3-sn-phosphatidate phosphohydrolase, EC 3.1.3.4) in the yeast Saccharomyces cerevisiae catalyzes the dephosphorylation of PA to form diacylglycerol and Pi. PA phsophatase plays a role in the regulation of phospholipid and triacylglycerol synthesis in S. cerevisiae. Two membrane- associated forms (45-kDa and 104-kDa) of PA phosphatase have been purified and characterized in our laboratory. In this grant application we propose to initiate a project to clone the structural genes for the 45-kDa and 104-kDa forms of PA phosphatase. We will use specific antibodies to the enzymes and lambda-ZAP cDNA expression and lambda-DASH genomic expression libraries. The cloned genes will be characterized and the essential nature of PA phosphatase will be addressed. %%% The enzyme phosphatidate (PA) phosphatase in the yeast Saccharomyces cerevisiae catalyzes the removal of phosphate from the phospholipid PA to form the neutral lipid diacylglycerol. This enzyme plays a role in the control of overall lipid synthesis in yeast and in animal cells. Two membrane-associated forms (45-kDa and 104-kDa) of PA phosphatase have been purified and characterized in our laboratory. In this grant application we propose to initiate a project to isolate the genes which encode for the 45-kDa and 104-kDa forms of PA phosphatase. We will use specific antibodies to the enzymes and DNA expression libraries in lambda viruses. Once isolated the genes for these enzymes will be characterized. The essential nature and role of these genes in yeast lipid synthesis and cell growth will be examined.

磷脂酸（PA）磷酸酶（3-sn-磷脂酸 磷酸水解酶，EC 3.1.3.4）在酵母酿酒酵母中 催化PA去磷酸化形成甘油二酯和Pi。 PA磷酸酶在调节磷脂和 S.啤酒。 双膜- PA磷酸酶的相关形式（45-kDa和104-kDa）已经被发现， 在我们的实验室中进行了纯化和表征。 在这份赠款中， 我们建议启动一个项目， 45-kDa和104-kDa形式PA的结构基因 磷酸酶。 我们将使用针对酶的特异性抗体， ZAP cDNA表达和DASH基因组表达 图书馆. 克隆的基因将被表征， PA磷酸酶的基本性质将得到解决。 %%% 酵母中的磷脂酸（PA）磷酸酶 酿酒酵母催化磷酸盐的去除， 磷脂PA形成中性脂质甘油二酯。 这 酶在控制整个脂质合成中起作用， 酵母和动物细胞。 两种膜结合形式（45-kDa 和104-kDa）的PA磷酸酶已被纯化和表征 在我们的实验室里。 在这份资助申请中，我们建议 启动了一个项目，分离编码45-kDa的基因， 和104-kDa形式的PA磷酸酶。 我们将使用特定的 针对λ中的酶和DNA表达文库的抗体 病毒 一旦分离出这些酶的基因， 表征了 这些基因的基本性质和作用， 将检测酵母脂质合成和细胞生长。