SBIR Phase I: Bioengineering probiotic yeast to mitigate methane emissions from cattle

SBIR 第一阶段：生物工程益生菌酵母可减少牛的甲烷排放

• 批准号: 2322126
• 负责人: Scott Collins
• 金额: $ 27.31万
• 依托单位: HOOFPRINT BIOME, INC.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2024
• 资助国家: 美国
• 起止时间: 2024-01-15 至 2024-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2322126&HistoricalAwards=false
• 关键词: SBIR; Phase; Bioengineering; probiotic; yeast

This Small Business Innovation Research (SBIR) Phase I project develops a technology that could drastically reduce the impact of livestock production on the environment. Methane emissions from livestock account for over 10% of global greenhouse gas emissions, which must be reduced significantly in order to meet climate change reduction targets. Simultaneously, profit margins in beef and dairy farming are slim, leaving farmers unable to adopt climate-friendly practices that are too costly. This product could increase the profitability of cattle farming while playing a key role in achieving net zero emissions from the food system.This team develops enzymes to be secreted from a probiotic yeast to eliminate methane emissions from cattle. The company has previously identified a set of enzymes that restrict methane production in the rumen of cattle. This project will further develop and package these enzymes in a probiotic yeast strain which has already been shown to increase milk yield by over 5%, thereby offering a dual benefit to the farmers. Project success requires a) screening an enzyme library to identify lead candidates for reduction of rumen methane, b) optimizing enzymes for further methane reduction, and c) demonstrating enzyme secretion and cost-efficient enzyme delivery by the probiotic yeast. Completion of these objectives will yield a product that will decrease cattle methane emissions by a minimum of 30%, and potentially by up to 98%, while simultaneously improving the health and productivity of cattle.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这个小型企业创新研究（SBIR）第一阶段项目开发了一种技术，可以大大减少畜牧业生产对环境的影响。畜牧业的甲烷排放量占全球温室气体排放量的10%以上，必须大幅减少，以实现气候变化减排目标。与此同时，牛肉和奶牛养殖的利润率很低，使农民无法采取成本太高的气候友好型做法。该产品可以提高养牛业的盈利能力，同时在实现食品系统的净零排放方面发挥关键作用。该团队开发了益生菌酵母分泌的酶，以消除牛的甲烷排放。该公司此前已经确定了一组限制牛瘤胃中甲烷产生的酶。该项目将进一步开发这些酶，并将其包装在益生菌酵母菌株中，该菌株已被证明可将牛奶产量提高5%以上，从而为农民提供双重利益。项目的成功需要a）筛选酶库以鉴定减少瘤胃甲烷的主要候选物，B）优化酶以进一步减少甲烷，以及c）证明益生菌酵母的酶分泌和具有成本效益的酶递送。完成这些目标将产生一个产品，将减少牛甲烷排放量的最低30%，并可能高达98%，同时提高牛的健康和生产力。这个奖项反映了NSF的法定使命，并已被认为是值得通过评估使用基金会的知识价值和更广泛的影响审查标准的支持。