Developmental Genetics of the Xenopus laevis src Genes

这项研究的目的是了解pp60src是如何 功能，以调节结构和生化特征， 非洲爪蟾卵母细胞。细胞骨架变化的性质 pp60src在卵母细胞中的表达 活动将被审查。酪氨酸的模式 产生激酶组成型pp60src的卵母细胞中的磷酸化 当这些卵母细胞重新进入细胞周期时， 孕酮治疗。激活卵母细胞的内源性 通过显微注射细胞周期蛋白 mRNA将用于检测MPF是否对此负责 基板图案的变化。免疫化学和生物化学 方法将用于确定pp60src中的更改是否 分布或修饰伴随着基质的变化 格局将使用定点诱变来尝试 定义pp60src的一部分，该部分介导响应， 孕酮

The goal of the proposed research is to understand how pp60src functions to regulate structural and biochemical features of the Xenopus oocyte. The nature of cytoskeletal changes caused by expression in oocytes of pp60src with constitutive kinase activity will be examined. The pattern of tyrosine phosphorylation in oocytes producing kinase-constitutive pp60src is altered when such oocytes are made to reenter the cell cycle by progesterone treatment. Activation of the oocyte's endogenous maturation promoting factor (MPF) by microinjection of cyclin mRNA will be used to examine whether MPF is responsible for this change in substrate pattern. Immunochemical and biochemical methods will be used to determine whether changes in pp60src distribution or modification accompany the change in substrate pattern. Site directed mutagenesis will be used in an attempt to define the portion of pp60src which mediates the response to progesterone.