BAC and YAC Libraries for Exploring Metazoan Origins

用于探索后生动物起源的 BAC 和 YAC 文库

• 批准号: 0208335
• 负责人: Robert Steele
• 金额: $ 6.62万
• 依托单位: University of California-Irvine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0208335&HistoricalAwards=false
• 关键词: BAC; YAC; Libraries; Exploring; Metazoan

0208335SteeleA complete description of the evolutionary history of multicellular animals will require detailed information on the content, organization, and regulatory mechanisms of the genomes of animals from phyla throughout the animal phylogenetic tree. Of particular importance for such studies are species that diverged early from the rest of the animals and unicellular organisms which are close relatives of animals. Features of the genomes of such organisms which are shared with more recently diverged animals would then be ones which were present in the ancestor of all modern animals. The goal of this project is to prepare and archive bacterial artificial chromosome libraries from Nematostella vectensis (a sea anemome which is a member of the early diverging animal phylum Cnidaria) and from the choanoflagellate Monosiga brevicollis, a unicellular organism closely related to multicellular animals. These libraries will provide important resources for addressing a variety of questions related to the origins, evolution, and development of multicellular animals. Such questions include: How did the Hox family of developmental regulatory genes evolve? When did various other developmental regulatory gene families appear during animal evolution? How closely related are choanoflagellates and multicellular animals, and what genes were necessary to make the transition from unicellularity to multicellularity? What happened at the genome level when nerves were added to multicellular animals? What cell adhesion and intercellular signaling molecules were present in the common ancestor of modern animals? Are cnidarian genes organized into operons, as suggested by the presence of spliced leader addition in this phylum? In addition to providing material for analysis of animal evolution, the libraries will facilitate the isolation of gene promoters, which will be essential for the development of functional assays in these organisms and for dissecting gene regulatory networks. The libraries generated by this project will be freely available to the research community. Information on the libraries and their availability will be provided through various means, including appropriate scientific newsgroups and web sites.

0208335完整描述多细胞动物的进化史需要从动物系统发育树的门中获取动物基因组的内容、组织和调控机制的详细信息。对这类研究特别重要的是早期从其他动物中分化出来的物种和与动物有近亲关系的单细胞生物。这些生物体的基因组特征与最近分化的动物共有，因此这些特征存在于所有现代动物的祖先中。本项目的目标是从Nematostella vectensis（一种海葵，是早期分化的动物刺胞门的成员）和从choanoflagellate Monosiga brevicollis（一种与多细胞动物密切相关的单细胞生物）制备和存档细菌人工染色体文库。这些图书馆将为解决与多细胞动物的起源，进化和发展有关的各种问题提供重要的资源。这些问题包括：Hox家族的发育调控基因是如何进化的？在动物进化过程中，其他各种发育调控基因家族是什么时候出现的？领鞭毛虫和多细胞动物的关系有多密切？从单细胞到多细胞的转变需要哪些基因？当神经被添加到多细胞动物中时，在基因组水平上发生了什么？在现代动物的共同祖先中存在什么样的细胞粘附和细胞间信号分子？刺胞动物的基因是否被组织成操纵子，就像这个门中存在剪接前导序列所暗示的那样？除了为动物进化分析提供材料外，文库还将促进基因启动子的分离，这对于在这些生物体中开发功能测定和解剖基因调控网络至关重要。该项目产生的图书馆将免费提供给研究界。将通过各种方式，包括适当的科学新闻组和网站，提供关于图书馆及其可利用性的信息。