Regulation and Function of the cut Locus in Drosophila

切割位点是一个大的果蝇基因， 各种组织的分化。 基因突变 将外部感觉器官转变为弦音器官， 也阻止了特殊翅缘刚毛的分化 就像昆虫的肾脏一样。 表达式也需要在头中， 角质层扩张的发生，一种程序化的行为。 基因转录部分上游的突变会干扰 适当的监管。 许多上游突变是 反转录转座子吉普赛的插入， 作为启动子和增强子之间的屏障 元素 cut与其他基因的相互作用以及cut基因的表达 产品在监管过程中导致差异化 将检查细胞类型。 削减的监管要素将是 通过它们调节报告基因的能力来鉴定，和 将进行实验来测试特定的基因， 在特定组织中表达cut需要哪些基因 哪些基因编码的蛋白质能直接与切口结合 监管要素。 切规的本土化 这些元素也将清楚地表明， 转座因子作为增强子之间的屏障， 发起人。

The cut locus is a large Drosophila gene that controls differentiation of a variety of tissues. Mutations of the gene transform the external sensory organs into chordotonal organs and prevent differentiation of particular wing margin bristles as well as the insect kidneys. Expression is also required in the head for the occurrence of cuticle expansion, a programmed behavior. Mutations upstream of the transcribed portion of the gene interfere with proper regulation. Many of the upstream mutations are insertions of the retrotransposon gypsy, which may interrupt expression by acting as a barrier between the promoter and enhancer elements. The interactions of cut with other genes and gene products in the process of regulation that leads to differentiation of cell type will be examined. The cut regulatory elements will be identified by their ability to regulate a reporter gene, and experiments will be carried out to test specific genes to discover what genes are required for cut expression in particular tissues and which ones encode proteins that bind directly to the cut regulatory elements. The localization of the cut regulatory elements will also demonstrate clearly whether the gypsy transposable element acts as a barrier between enhancers and promoters.