Expression and Biochemical Characterization of Arabidopsis Calmodulin Isoforms

本项目旨在阐明细胞内 信号通路，涉及钙在转导 激素和环境刺激。 当前 该项目的重点是信号转导途径的一部分， 涉及钙和钙调蛋白之间的相互作用， 随后激活特定的靶酶。 初步 该项目的工作建立了四个不同的存在 钙调素同种型由至少六种不同的基因组DNA编码 来自拟南芥的序列。 钙调蛋白的多样性 同种型在植物或动物界都是前所未有的， 初步的系统发育分析表明， 可能是拟南芥或十字花科植物所独有的。 这些 这些发现导致了一种假设，即不同的拟南芥 钙调素异构体已经进化到优化 钙调素与特异性靶酶，特别是靶 与细胞膜相关的酶。 %%% 这个项目是针对细胞内信号 在高等植物中的转导途径。 目前只有 对植物界的这些过程有一个普遍的了解。 初步数据表明存在多种亚型 钙结合诱导蛋白，钙调蛋白，在拟南芥中， thaliana. 这一意想不到的结果可能是重要的基础 植物和其他生物在生物学机制上的差异 信号接收和传导。

This project is aimed toward the elucidation of intracellular signalling pathways that involve calcium in the transduction of hormonal and environmental stimuli in higher plants. The current project focuses on the part of the signal transduction pathway that involves the interaction between calcium and calmodulin, and the consequent activation of specific target enzymes. The preliminary work for this project established the existence of four different calmodulin isoforms encoded by at least six different genomic DNA sequences from Arabidopsis thaliana. This diversity of calmodulin isoforms is unprecedented in either the plant or animal kingdoms, and preliminary phylogenetic analyses suggest that this arrangement may be unique to Arabidopsis or to the crucifer family. These findings have led to the hypothesis that the different Arabidopsis calmodulin isoforms have evolved to optimize the interaction of calmodulin with specific target enzymes, particularly target enzymes associated with membranes. %%% This project is directed toward intracellular signal transduction pathways in higher plants. At present, there is only a general understanding of these processes in the plant kingdom. The preliminary data demonstrate the existence of multiple isoforms of the calcium-binding elicitor protein, calmodulin, in Arabidopsis thaliana. This unexpected result may underlie significant differences between plants and other organisms in the mechanisms of signal reception and transduction.