RAS Proto-Oncogene Activity in Corpus Lutem Function

最近发现，在大鼠中，卵巢中含有的一种特殊蛋白质K-ras p21蛋白的数量反映了黄体中黄体酮的分泌模式。此外，体外将该蛋白的抗体掺入黄体细胞后，黄体细胞的黄体酮分泌明显减少，从而证明该蛋白在黄体细胞产生/分泌黄体酮中起作用。本课题的目的是确定K-ras p21蛋白影响黄体黄体酮分泌的具体方式，并确定该p21蛋白是否在黄体其他激素的分泌中起作用。K-ras p21蛋白存在于所有哺乳动物细胞中。因此，所提出的研究结果将对卵巢激素分泌机制的理解以及我们对所有哺乳动物细胞对激素和生长因子的反应方式的理解产生重大影响。

It was recently discovered that, in the rat, the amounts of a particular protein, the K-ras p21 protein, contained in the ovary mirror the pattern of secretion of progesterone from the corpus luteum. Furthermore, when an antibody against this protein was incorporated into luteal cell in vitro, progesterone secretion from these cells was significantly decreased, thus demonstrating that this protein plays a role in the production/secretion of progesterone by the luteal cell. It is the objective of this proposal to determine the specific way in which the K-ras p21 protein affects luteal progesterone secretion and to determine whether this p21 protein plays a role in the secretion of other hormones from the luteum. The K-ras p21 protein is present in all mammalian cells. Thus, the results of the proposed studies will have a significant impact upon the understanding of ovarian hormone secretory mechanisms as well as our understanding of the ways in which all mammalian cells respond to hormones and growth factors.