U.S.-France Cooperation: Tissue-specific Transcription Fac-tor GHF-1/Pit-1 and the Ets-1 Proto-oncogene in Differenti- ation and Ras Oncogene Signaling Pathways

美法合作：分化和Ras癌基因信号通路中的组织特异性转录因子GHF-1/Pit-1和Ets-1原癌基因

• 批准号: 9415882
• 负责人: A Gutierrez-Hartmann
• 金额: $ 1.8万
• 依托单位: University of Colorado at Denver
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-03-15 至 2000-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9415882&HistoricalAwards=false
• 关键词: U.S.; France; Cooperation; Tissue; specific

This three-year award will support U.S.-France cooperative research in biochemical genetics among several American and French researchers at the University of Colorado and the Laboratory for Molecular Genetics, French National Center for Scientific Research, in Strasbourg, France. The investigators are Arthur Gutierrez-Hartmann, Andrew Bradford and Bohdan Wasylyk. The objectives of their research are to define regions of the molecules Ets-1 protoocogene and GHF-1/Pit-1 and their role in cellular differentiation and malignant transformation. They will perform site-directed mutagenesis experiments in order to define regions of the two proteins that are involved in mediating their interaction. The U.S. investigators bring to this collaboration their expertise in hormonal regulation of gene expression. This is complemented by the French investigator's expertise in the Ets molecules and takes advantage of French techniques in generating on site Ets mutants. The results will advance our understanding of molecular mechanisms common to all cell types, healthy and malignant, and cell-specific responses.

这个为期三年的奖项将支持美国和法国科罗拉多大学以及位于法国斯特拉斯堡的法国国家科学研究中心分子遗传学实验室的几名美国和法国研究人员在生化遗传学方面的合作研究。调查人员是Arthur Gutierrez-Hartmann， Andrew Bradford和Bohdan Wasylyk。他们的研究目标是确定Ets-1原基因和GHF-1/Pit-1分子的区域及其在细胞分化和恶性转化中的作用。他们将进行定点诱变实验，以确定这两种蛋白质中参与介导它们相互作用的区域。美国研究人员将他们在激素调节基因表达方面的专业知识带到这次合作中。法国研究人员在et分子方面的专业知识补充了这一点，并利用法国技术在现场产生et突变体。结果将促进我们对所有细胞类型，健康和恶性以及细胞特异性反应的分子机制的理解。