U.S.-France Cooperation: Tissue-specific Transcription Fac-tor GHF-1/Pit-1 and the Ets-1 Proto-oncogene in Differenti- ation and Ras Oncogene Signaling Pathways

这项为期三年的奖励将支持美国-法国在科罗拉多大学和位于法国斯特拉斯堡的法国国家科学研究中心分子遗传学实验室的几位美国和法国研究人员之间进行的生化遗传学合作研究。 调查人员是亚瑟古铁雷斯-哈特曼、安德鲁·布拉德福德和博赫丹·瓦西雷克。 他们的研究目标是确定Ets-1原癌基因和GHF-1/Pit-1分子的区域及其在细胞分化和恶性转化中的作用。 他们将进行定点诱变实验，以确定参与介导其相互作用的两种蛋白质的区域。 美国研究人员将他们在基因表达的激素调节方面的专业知识带到了这次合作中。法国研究人员在Ets分子方面的专业知识对此进行了补充，并利用法国技术在现场产生Ets突变体。 这些结果将促进我们对所有细胞类型，健康和恶性细胞以及细胞特异性反应的共同分子机制的理解。

This three-year award will support U.S.-France cooperative research in biochemical genetics among several American and French researchers at the University of Colorado and the Laboratory for Molecular Genetics, French National Center for Scientific Research, in Strasbourg, France. The investigators are Arthur Gutierrez-Hartmann, Andrew Bradford and Bohdan Wasylyk. The objectives of their research are to define regions of the molecules Ets-1 protoocogene and GHF-1/Pit-1 and their role in cellular differentiation and malignant transformation. They will perform site-directed mutagenesis experiments in order to define regions of the two proteins that are involved in mediating their interaction. The U.S. investigators bring to this collaboration their expertise in hormonal regulation of gene expression. This is complemented by the French investigator's expertise in the Ets molecules and takes advantage of French techniques in generating on site Ets mutants. The results will advance our understanding of molecular mechanisms common to all cell types, healthy and malignant, and cell-specific responses.