Hormonal Regulation of Cytoplasmic Ca2+ in the Cereal Aleurone

谷物糊粉中细胞质 Ca2 的激素调节

• 批准号: 9206692
• 负责人: Douglas Bush
• 金额: $ 51万
• 依托单位: Rutgers University New Brunswick
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-07-01 至 1998-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9206692&HistoricalAwards=false
• 关键词: Hormonal; Regulation; Cytoplasmic; Ca2+; Cereal

This project is aimed toward the elucidation of intracellular signalling pathways that involve calcium in the transduction of hormonal and environmental stimuli in higher plants. The project is focused on the necessity and sufficiency of changes in cytosolic calcium activity in aleurone cells from wheat seeds after hormonally- or environmentally-induced germination. The early signal transduction events in the seed culminate in a change in gene expression in the aleurone cells. Cytosolic calcium activity changes will be determined in individual cells fluorometrically, and changes in calcium channel activity that underlie the redistribution of calcium will be assessed by patch clamp studies. Finally, the timing of the changes in gene expression will be compared with the timing of shifts in cytosolic calcium activity. The thrust of the project is to link transient shifts in cytosolic calcium activity with specific changes in channel activity and with changes in transcription and translation. %%% This study is directed toward intracellular signalling pathways in higher plants. In the process of seed germination, much is known about hormonal activation of the aleurone cells in the seed and much is known about the changes in gene expression that follow several hours after hormonal stimulation, but the intermediary steps of hormonal reception and intracellular signal transduction that lead to altered transcription and translation remain elusive. This project will provide significant, new information on the involvement of calcium in the cascade that culminates in altered gene expression in developing seeds of wheat.

本项目旨在阐明细胞内 信号通路，涉及钙在转导 激素和环境刺激。 项目 的必要性和充分性， 小麦种子胚乳糊粉层细胞钙活性 植物或环境诱导的发芽。 早期 种子中的信号转导事件最终导致 糊粉层细胞中的基因表达。 胞浆钙活性 将通过荧光测定法确定单个细胞中的变化， 以及钙通道活性的变化， 通过膜片钳研究评估钙的再分布。 最后，基因表达变化的时间将是 与胞质钙活性变化的时间相比。 该项目的主旨是将细胞质中的瞬时变化与细胞内的细胞质中的瞬时变化联系起来。 钙活性与通道活性的特定变化， 转录和翻译的变化。 %%% 这项研究是针对细胞内信号通路 在高等植物中。 在种子发芽的过程中， 已知种子中糊粉层细胞的激素激活 我们对随后的基因表达变化也有很多了解， 激素刺激后几个小时，但中介 激素接受和细胞内信号转导的步骤 导致转录和翻译改变的基因仍然是难以捉摸的。 该项目将提供重要的新信息， 钙参与级联反应，最终导致改变 小麦种子发育过程中的基因表达。