Relationships Among Percid Fishes (Teleostei: Percidae) as Evidenced by Mitochondrial DNA Sequence Data

线粒体 DNA 序列数据证明鲈鱼（Teleostei：鲈科）之间的关系

• 批准号: 9207600
• 负责人: Edward Wiley
• 金额: --
• 依托单位: University of Kansas Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1994-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9207600&HistoricalAwards=false
• 关键词: Relationships; Among; Percid; Fishes; Teleostei

The objectives of this proposal are to test the evolutionary relationships among selected species of the genus Etheostoma and to test the monophyly of this genus relative to its closest relatives, Percina and Crystallaria. These genera comprise the second largest group of North American freshwater fishes and the majority of species in the family Percidae. Etheostoma is the largest genus (114 species compared to 34 species of Percina and one species of Crystallaria). However, the monophyly of the genus is not well established and numerous problems exist concerning the relationships among the 18 subgenera. Polymerase chain reaction and direct sequencing protocols will be used to amplify and sequence parts of the 12S ribosomal gene (800 base pairs) and a segment of the cytochrome-b genes (450 base pairs) of 15 species of Etheostoma, four species of Percina, and two outgroups, Crystallaria asprella, and Stizostedion canadense. This will allow me to perform a preliminary test of the monophyly of Etheostoma, explore the possibility that some Etheostoma are actually more closely related to Percina, and arrive at some preliminary conclusions regarding the relationships among selected species groups of Etheostoma. Phylogenetic analysis employing several parsimony criteria will be used to test alternate hypotheses of relationship. Resolution of the relationships among darters will lead to a better understanding of the origin and diversification of the North American freshwater fish fauna and enhance our ability to study the speciation, historical ecology, and comparative behavior of this speciose group.

本建议的目的是测试的进化关系之间的选择属Etheostoma的物种，并测试该属相对于其最近的亲戚，鲈和Crystallaria的单系性。这些属包括北美淡水鱼的第二大群体和Perkalae家族的大多数物种。镖鲈属是最大的属（114种，鲈属有34种，水晶属有1种）。然而，该属的单系性并没有很好地建立，18个亚属之间的关系存在许多问题。聚合酶链反应和直接测序方案将用于扩增和测序部分的12 S核糖体基因（800个碱基对）和细胞色素-b基因（450个碱基对）的15个物种的镖鲈，四个物种的鲈，和两个外类群，Crystallaria asprella，和Stizostedion canadense。这将使我能够进行初步测试的单系的镖鲈，探索的可能性，一些镖鲈实际上是更密切相关的鲈，并得出一些初步结论，关于镖鲈的选定物种群体之间的关系。采用几个简约标准的系统发育分析将用于测试关系的替代假设。镖鲈之间的关系的决议将导致更好地了解北美淡水鱼类区系的起源和多样化，提高我们的能力，研究物种形成，历史生态学和比较行为的这一speciose组。