Spermatogenesis in Pteridophytes: Ultrastructure, Differentiation and Phylogeny

蕨类植物的精子发生：超微结构、分化和系统发育

• 批准号: 9207646
• 负责人: Karen Renzaglia
• 金额: $ 19.5万
• 依托单位: East Tennessee State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-01 至 1996-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9207646&HistoricalAwards=false
• 关键词: Spermatogenesis; Pteridophytes; Ultrastructure; Differentiation; Phylogeny

Comparative data on spermatogenesis spanning a range of pteridophytes will be collected. Emphasis will be placed on elucidating the ultrastructural details of spermiogenesis and the fine structure of the mature sperm cell. The role of three cytoskeletal elements - microtubules, actin and centrin - in the differentiation process will be evaluated by fluorescent labelling and immunogold-localization. These data will be compared with data already available on the bryophytes. Both taxa of spermatophytes that have flagellated sperm and two species of charaphytes will also be examined. The bryophytes, algae, and seed plants will be used as outgroups in a cladistic analysis of the data. The purpose of this study is resolve phylogenetic relationships among early pteridophytes and between them and the algal, bryophyte and seed plant lineages. The major dichotomies in early diversification of archegoniate groups remain as speculative today as they did before the advent of biological electron microscopy. This study should enable the resolution of early lines of diversification from the green algae to the major extant groups of land plants.

关于精子发生的比较数据， 将收集蕨类植物。 重点将放在 阐明精子发生的超微结构细节， 成熟精子细胞的精细结构。 三人的作用 细胞骨架元件-微管，肌动蛋白和中心蛋白-在 分化过程将通过荧光检测来评估。 标记和免疫金定位。 这些数据将 与已有的关于Brittites的数据相比。 两 有鞭毛精子的种子植物分类群和两个 还将检查charaphytes的种类。 布列塔尼人， 藻类和种子植物将被用作分支系统中的外类群 数据分析。 本研究的目的是解决 早期蕨类植物之间的系统发育关系和 他们和藻类，briterite和种子植物谱系。 主要 颈卵器类群早期分化的二分法 今天仍然像他们在出现之前一样投机。 生物电子显微镜 这项研究将使 从绿色藻类中分离出早期的多样化品系 陆地植物的主要现存群体。