Molecular phylogenetic analyzes of pteridophytes based on rbcL sequence comparison

基于rbcL序列比较的蕨类植物分子系统发育分析

• 批准号: 04404003
• 负责人: IWATSUKI Kunio
• 金额: $ 19.84万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04404003/
• 关键词: pteridophyte; rbcL; nucleotide sequence; molecular tree; Aspleniaceae; Hymenasplenium; systematics; evolution; 系統; 分子系統; ホウビシダ節

In this study, we first developed widely-applicable method in amplifying and sequencing rbcL gene from pteridophytes. We tested many kinds of primers for PCR amplification of rbcL fragments and got universal primers for pteridophytes which is also applicable for seed plants. Performing this method with DNA sequencer which we bought by this grant, we became able to determine about 1200bp sequence of rbcL of one wild pteridophyte species in 2.5 days. We selected one species each from 32 family, 107 genera, that cover whole variation of pteridophytes, and determined their rbcL sequences. Based on them, we constructed molecular tree and global phylogenetic relationships in fern families were revealed. In this study, we also analyzed smaller groups of ferns (epecially Aspleniaceae) using the same molecular method. Aspleniaceae is one of the largest family of ferns, containing more than 700 species. Its Intrafamiliar phylogeny was poorly known. We determined rbcL sequences from 61 species of Aspleniaceae. We elucidated global phylogenetic relation in the family as well sd smaller groups in the family : genus Hymenasplenium (20 species) and H.obliquissimum species (17 samples) complex. Molecular phylogenetic analyzes based on rbcL sequencing was valid from inter-family to intra-species levels.

在本研究中，我们首次建立了一种广泛适用于蕨类植物rbcL基因扩增和测序的方法。我们测试了多种引物用于rbcL片段的PCR扩增，得到了适用于蕨类植物的通用引物，也适用于种子植物。利用这笔资金购买的DNA测序仪，我们在2.5天内测定了一种野生蕨类植物rbcL的约1200bp序列。从覆盖整个蕨类植物变异的32科107属中各选1种，测定其rbcL序列。在此基础上，构建了蕨类植物分子树，揭示了蕨类植物的全球系统发育关系。在这项研究中，我们还使用相同的分子方法分析了较小的蕨类植物（特别是Aspleniaceae）。蕨科是蕨类植物中最大的科之一，有700多种。其内部熟悉的系统发育鲜为人知。对61种阿连科植物的rbcL序列进行了测定。我们对该科的全球系统发育关系以及该科中较小的类群：Hymenasplenium属（20种）和H.obliquissimum种（17个样本）复合体进行了研究。基于rbcL序列的分子系统发育分析从科间到种内水平都是有效的。

项目成果

Murakami,N.: "Systematics and Evolutionary Biology of the Fern Genus Hymenasplenium(Aspleniaceae)" J.Plant Res.108(掲載予定). (1995)

Murakami, N.：“蕨属 Hymenasplenium（Aspleniaceae）的系统学和进化生物学”J.Plant Res.108（待出版）。

Hasebe,M.,et al.: "Phylogenetic relationships of Ferns deduced from rbcL gene sequence." J.Mol.Evol.37(5). 476-482 (1993)

Hasebe,M.,et al.：“从 rbcL 基因序列推导出蕨类植物的系统发育关系。”

Hasebe et al.: "A global analysis of fern phylogeny based on rbcL nucleotide sequences." Amer.Fern J.85(掲載予定). (1995)

Hasebe 等人：“基于 rbcL 核苷酸序列的蕨类植物系统发育的整体分析。”Amer.Fern J.85（即将出版）。

Hasebe, M.et al.: "rbcL gene sequences provide evidence for the evolutionary lineages of leptosporangiate ferns." Proc.Natl.Acad.Sci.USA. Vol.91. 5730-5734 (1994)

Hasebe, M.等人：“rbcL 基因序列为薄孢子蕨类植物的进化谱系提供了证据。”

Hasebe, M.et al.: "A global analysis of fern phylogeny based on rbcL nucleotide sequences. (In press)" Amer.Fern J.Vol.85. (1995)

Hasebe, M.et al.：“基于 rbcL 核苷酸序列的蕨类植物系统发育的全球分析。（正在出版）”Amer.Fern J.Vol.85。