Specific Compartmentalization of Proteins in Oogenesis

卵子发生过程中蛋白质的特定区室化

• 批准号: 9208018
• 负责人: Gary Wessel
• 金额: $ 27万
• 依托单位: Brown University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1996-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9208018&HistoricalAwards=false
• 关键词: Specific; Compartmentalization; Proteins; Oogenesis

The developmental success of an early embryo depends fundamentally on the efficacy of oogenesis. Developing oocytes are geared almost entirely to the segregation and storage of newly synthesized macromolecules. Dr. Wessel will examine the ontogeny of two classes of storage organelles in oocytes of the sea urchin - vesicles storing extracellular matrix molecules that are destined for the embryonic blastocoel, and cortical granules that are exocytosed during the fertilization reaction. Both of these organelles originate early in oogenesis and their contents are each secreted, yet each organelle contains a discreet, non- overlapping population of proteins in distinct membrane bound vesicles. Dr. Wessel will examine the ontogeny of these organelles using cDNA and antibody probes that he has made to specific constituent proteins. The ultimate goal is to understand the mechanism of specific protein "stockpiling" that developing oocytes use to facilitate the commitment of their embryos to a program of rapid, independent development.

早期胚胎的发育成功取决于 从根本上讲是关于卵子发生的功效。卵母细胞发育 几乎完全是为了隔离和储存 新合成的大分子。韦塞尔医生会检查 卵母细胞中两类储存细胞器的个体发育 海胆-储存细胞外基质分子的囊泡， 是胚胎囊胚腔和皮质颗粒的目的地 在受精反应过程中被细胞外排出。两 这些细胞器起源于卵子发生的早期， 每个人都有自己的秘密，但每个人都有自己的秘密。 不同膜结合的蛋白质重叠群体 囊泡韦塞尔博士将研究这些 细胞器使用cDNA和抗体探针， 特定的组成蛋白质。最终目的是了解 特定蛋白质“囤积”的机制 卵母细胞用于促进胚胎向 快速、独立的发展。