Salivary Gland Genetic Markers

唾液腺遗传标记

• 批准号: 9208110
• 负责人: Steven Beckendorf
• 金额: $ 32万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-01 至 1996-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9208110&HistoricalAwards=false
• 关键词: Salivary; Gland; Genetic; Markers

In Drosophila embryos, salivary glands arise from a thickened placode of cells in the ventral part of the labial segment. After the placode forms, its cells are invaginated to form a simple tubular gland. Within three hours the cells of the gland are already differentiated and producing abundant, secreted products. This simple example of organogenesis provides opportunities to examine the interaction between genes that establish the overall pattern of the embryo and those that direct tissue specific development. Dr. Beckendorf's experiments have focussed on the role of embryonic pattern forming genes in choosing the location of the salivary placodes and initiation salivary gland development. The goal of the current proposal is to identify additional genes that act downstream from the pattern forming genes to direct the morphogenesis and differentiation of the glands. Three independent approaches will be used to identify these new genes: analyzing enhancer trap lines, screening deficiency mutant embryos for salivary defects, and searching for downstream targets of the transcription factor encoded by the for head gene. These methods should identify a new set of genes that will help him characterize specific steps in salivary development. Because of the short time between initial determination and differentiation, he hopes eventually to identify many, if not all, of the intervening steps in salivary development.

在果蝇胚胎中，唾液腺是由唇节腹侧增厚的细胞组成的。基因座形成后，其细胞内陷形成一个简单的管状腺。在三个小时内，腺体的细胞已经分化并产生大量的分泌产物。这个简单的器官发生的例子提供了一个机会来研究建立胚胎整体模式的基因和指导组织特定发育的基因之间的相互作用。贝肯多夫博士的实验集中在胚胎模式形成基因在选择唾液腺基板位置和开始唾液腺发育中的作用。目前的建议的目标是确定在模式形成基因下游的其他基因，以指导腺体的形态发生和分化。三种独立的方法将用于鉴定这些新基因：分析增强子陷阱系，筛选唾液缺陷缺陷突变胚胎，以及寻找由for head基因编码的转录因子的下游靶标。这些方法应该确定一组新的基因，这将有助于他描述唾液发育的具体步骤。由于从最初的确定到分化之间的时间很短，他希望最终能够确定唾液发育过程中的许多干预步骤，如果不是全部的话。