Assessing the genetic basis of arboviral salivary gland escape barriers in Aedes aegypti.

评估埃及伊蚊虫媒病毒唾液腺逃逸障碍的遗传基础。

• 批准号: 9392524
• 负责人: WILLIAM C. BLACK
• 金额: $ 22.8万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-12-02 至 2019-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9392524
• 关键词: Aedes; Affect; African; Alphavirus; American; Arboviruses; Asian Americans; Asians; Biological Assay; Breeding; Bunyaviridae; Chest; Culicidae; Data; Dengue Hemorrhagic Fever; Dengue Virus; Disease Outbreaks; Dose; Environmental Risk Factor; Experimental Designs; Female; Flavivirus; Future; Genes; Genetic; Genetic Markers; Genetic Variation; Genotype; Geographic state; Gland; Head; Heritability; Immune system; Indian Ocean; Infection; Investigation; Leg; Malaysia; Measures; Methods; Mexican; Mexico; Midgut; Mothers; Nucleic Acids; Oils; Organ; Partner in relationship; Pharmacologic Substance; Play; Population; Quantitative Genetics; RNA; Reporting; Research Personnel; Role; Saliva; Salivary Glands; Senegal; Serotyping; South African; South America; Sucrose; Technology; Testing; Time; Tissues; Titrations; Transgenic Organisms; Variant; Viral; Virus; Virus Diseases; Work; Yellow Fever; ZIKV disease; Zika Virus; base; chikungunya; design; male; novel strategies; offspring; tissue culture; tool; transmission process; vector; viral RNA; viral transmission

PROJECT SUMMARY / ABSTRACT The salivary gland is the organ that ultimately transmits an arbovirus from an infected mosquito into her vertebrate host. Salivary gland infection barriers (SGIBs) and salivary gland escape barriers (SGEBs) determine whether the saliva of a mosquito will contain sufficient virus to overcome the vertebrate immune system. SGIBs are manifest as the absence of detectable virus in the salivary glands of mosquitoes that have a disseminated viral infection (as determined by bioassay of pulled legs or smashed heads). There are large numbers of studies that document SGIBs for bunyaviruses and flaviviruses. SGEBs on the other hand are manifest as the absence of detectable virus in the saliva of mosquitoes that have infected salivary glands (as determined by bioassay of dissected glands). In contrast to SGIBs, there are very few studies that document SGEBs in mosquitoes and those have only examined bunyaviruses. Furthermore these SGEB studies were not performed in genetically “tractable” mosquito species that can be easily colonized from the field, reared in the lab, crossed as single pairs and have an abundance of genetic markers. Because of this deficiency we have little understanding of whether there is a genetic basis for SGEBs or whether they are random, subject to a few or many “environmental” factors. The results of this study will indicate whether there are genetic factors in the mosquito which, if manipulated through transgenics or pharmaceuticals, could interfere with the ability of the mosquito to transmit an arbovirus. Alternatively if mosquito genetics accounts for very little of the ability of the mosquito to transmit arboviruses then this is unlikely to be a fruitful approach. PI Black's earlier work on the quantitative genetics of DENV-2 in Ae. aegypti midgut infection and escape barriers indicated that 40% of the variation was due to genetic effects and 60% was due to environmental effects. To date, many genes have been identified that affect tissue barriers to DENV-2 infections but many environmental effects (including viral genotype) have also been identified. Will the same hold true from SGIBs and SGEBs?

项目摘要/摘要

项目成果

The Genetic Basis for Salivary Gland Barriers to Arboviral Transmission.

• DOI: 10.3390/insects12010073
• 发表时间: 2021-01-15
• 期刊: Insects
• 影响因子: 3
• 作者: Sanchez-Vargas I;Olson KE;Black WC 4th
• 通讯作者: Black WC 4th