Environmental and Genetic Effects on Root Structure in Wild and Cultivated Lettuce (Lactuca spp.)

环境和遗传对野生和栽培生菜（Lactuca spp.）根结构的影响

• 批准号: 9208131
• 负责人: Louise Jackson
• 金额: $ 1.95万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-11-15 至 1994-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9208131&HistoricalAwards=false
• 关键词: Environmental; Genetic; Effects; Root; Structure

The objective of this proposal is to clarify factors influencing morphological variation in plant roots by examining genotypic differences in root system structure and responses to soil nitrogen (N) and water. The infertile wild and cultivated accessions of lettuce (Lactuca serriola and L. sativa) will provide a devierse set of germplasm adapted to a wide range of soil water and nutrient regimes. Genotypic differences in root system structure and plastic responses to enriched soil microsites will be studied using root topological methods and growth rate measurements. Ability to extract water, ammonium, and nitrate from enriched microsites will be studied using the root topological method, growth rates, measurements of plant water balance including both direct (stomatal conductance, leaf water potential) and indirect measurements (stable carbon isotope discrimination), and short-term labeling with the stable isotope, 15N. Improved understanding of environmental effects on root growth, including differences in response among genotypes, will provide a basis for improving water and N absorption of cultivated lettuce, a shallow-rooted crop with high demand for fertilizer N and irrigation inputs.

本提案的目的是澄清影响 通过检测基因型的植物根的形态变化 根系结构差异及其对土壤氮素的响应 (N)和水 不育的野生和栽培品种， 莴苣（Lactuca serriola和L. sativa）will provide a devierse 一套适应广泛土壤水分和养分的种质 政权。 根系结构的基因型差异， 将使用以下方法研究富集土壤微区的塑性反应： 根拓扑方法和生长速率测量。 能力 从富集的微站点中提取水、铵和硝酸盐， 使用根拓扑方法进行研究，增长率， 植物水分平衡的测量，包括直接（气孔） 电导率、叶水势）和间接测量 （稳定碳同位素鉴别）和短期标记 稳定同位素15N 更好地了解 环境对根系生长的影响，包括 基因型之间的反应，将提供基础，改善水 生菜是一种浅根作物， 对肥料氮和灌溉投入的高需求。