Stability of HIV Viral RNA in Environmental Conditions

这是一个支持研究的奖项，旨在确定在不同的环境条件下，与人类免疫缺陷病毒(HIV)同源的病毒核酸在不同环境条件下的稳定性，这些环境条件来自家庭污水处理厂的初级、二级和最终流出物，使用聚合酶链式反应方法检测被检测样本中的病毒核糖核酸的自来水和其他水溶液。研究人员计划在病毒失去感染细胞培养物的能力后，确定这种核酸持续的时间。这项建议是在NSF 89-85，探索性研究小额赠款(SGER)中概述的条件下提交给NSF的。先前的研究结果表明，废水浓缩液中存在与人类免疫缺陷病毒(HIV)“同源”的核酸，这表明在该病毒感染细胞培养的能力丧失后，确定这些核酸是否被降解的重要性。反过来，这将有助于评估通过环境媒介传播传染性病毒的可能性。

This is an award to support research to determine the stability of viral nucleic acids homologous to the Human Immunodeficiency Virus (HIV) under differing environmental conditions as encountered in primary, secondary and final effluents from domestic wastewater treatment plants, tap water and other aqueous fluids using the polymerase chain reaction method for detection of viral ribonucleic acid in samples being examined. The investigators plan on determining the duration of the persistence of this nucleic acid after the virus has lost its ability to infect cell cultures. This proposal was submitted to NSF under conditions outlined in NSF 89-85, Small Grants for Exploratory Research (SGER). Results of prior studies reporting the presence of nucleic acids "homologous" to the Human Immunodeficiency Virus (HIV) in wastewater concentrates suggest the importance of determining whether these nucleic acids are degraded after the ability of this virus to infect cell cultures has been lost. This, in turn, would assist in assessing the potential of infectious viral transmission through environmental media.