500MHz NMR for Natural Products and Biopolymers

9217481 Nakanishi作为哥伦比亚化学系研究设施持续维护的一部分，本提案寻求购买500 MHz多核NMR光谱仪。 在该部，有几个团体需要这种设备。 Nakanishi已经阐明了160多种生物活性化合物的结构，其中许多化合物不稳定，只能以微克的数量获得。 近年来，大多数属于这一类化合物的NMR研究已经在其他地方进行，因为该部门的仪器不足。 然而，他的小组最近进行的几乎所有工作的性质，即，在生物聚合物和超微小尺度结构测定中，使得现场高场NMR仪器的可用性绝对必要。 他的团队专注于光谱方法在结构研究中的发展和应用;拟议中的仪器将最终使他能够在哥伦比亚这里做重要的核磁共振工作。 我们最近增加了两名专门研究生物聚合物的年轻教师，Horne教授和McDermott教授，该仪器对他们的工作至关重要。 具体地说，McDermott的工作涉及配体-蛋白质与大蛋白质受体的相互作用，例如来自光合反应中心的可溶性结构域，使用固态和溶液NMR。 哥伦比亚现有的仪器既不充分，又过于频繁地使用，使她无法在这里继续这些研究的解决方案。 Horne在DNA三螺旋形成领域非常活跃，也在RNA-蛋白质相互作用领域工作。 Still、Breslow、Katz和Turro教授的研究工作受到了阻碍，因为他们实际上放弃了某些激励系统的结构分析，只是因为这些分析不能很好地解决过时的设备。 合成有机和无机组的其他成员，例如Gilber t Stork和其他人，也将在研究需要时使用该仪器。

9217481 Nakanishi As part of an ongoing maintenance of the Research Facilities in the Department of Chemistry at Columbia, this proposal seeks the purchase of a 500 MHz multinuclear NMR Spectrometer. In this Department such equipment is needed by several groups. Nakanishi has elucidated the structures of over 160 bioactive compounds, many of them unstable and available in only microgram quantities. In recent years most NMR studies belonging to compounds in this category have been performed elsewhere because of inadequate instrumentation in this department. However, the nature of almost all the work being conducted in his group recently i.e., in biopolymers and the ultraminuscule scale structure determinations, makes the availability of an on-site high- field NMR instrument absolutely essential. His group has focused on the development and application of spectroscopic methods to structural studies; the proposed instrument will finally enable him to do the important NMR work here at Columbia. We have recently added two young faculty members who specialize in biopolymers, Professors Horne and McDermott, and the instrument will be essential for their work. Specifically McDermott's work has concerned ligand- protein interactions with large protein receptors, such as soluble domains from photosynthetic reaction centers, using both solid state and solution NMR. Existing instrumentation at Columbia is both inadequate and too heavily used for her to continue the solution state portion of these studies here. Horne has been very active in the area of triple helix formation of DNA, and is also working in the area of RNA-protein interactions. The research efforts of Professor Still, Breslow, Katz and Turro have been hampered by the fact that they have effectively abandoned structural analyses of certain exciting systems only because they are not well resolved on antiquated equipment. Other members of the synthetic organic and inorganic group, for example, Gilber t Stork, and others, will also make use of the instrument as research requires.