Regulation of ribosomal RNA synthesis by fructose-1, 6-biophosphate

1, 6-生物磷酸果糖对核糖体 RNA 合成的调节

• 批准号: 9218196
• 负责人: Carol Gross
• 金额: $ 5万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-01-15 至 1993-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9218196&HistoricalAwards=false
• 关键词: Regulation; ribosomal; RNA; synthesis; fructose

Modulating the level of protein biosynthetic machinery in response to growth conditions is of utmost importance to every living cell. In E. coli, two different regulatory systems couple the rate of ribosomal RNA (rRNA) synthesis to growth rate. However, the actual mechanism(s) linking the central metabolism of the cell to the rate of rRNA synthesis remain completely unknown. The observation that increased amounts of fructose 1,6 biphosphate (FbP) preferentially inhibits in connecting central metabolism regulation of rRNA synthesis. The development of a crude transcription-translation system in which FbP specially inhibits rRNA synthesis provides an experimental system for dissecting this effect. Experiments to determine whether RNA polymerase alone or additional factors in the crude extract are needed for inhibition will determine the target of FbP action. Genetic analysis of this interaction will establish the growth conditions under which the regulatory circuit involving FbP is operative. %%% This project offers a chance to understand a molecular circuit that couples rRNA synthesis to carbon metabolism, a problem of central importance to every living organism that has not been elucidated in any experimental system.

根据生长条件调节蛋白质生物合成机制的水平对于每个活细胞至关重要。 在大肠杆菌中，两种不同的调节系统将核糖体 RNA (rRNA) 合成速率与生长速率结合起来。 然而，将细胞的中央代谢与 rRNA 合成速率联系起来的实际机制仍然完全未知。 观察发现，果糖 1,6 二磷酸 (FbP) 量的增加优先抑制 rRNA 合成的中枢代谢调节。 FbP 特异性抑制 rRNA 合成的粗转录-翻译系统的开发为剖析这种效应提供了一个实验系统。 确定是否需要单独的 RNA 聚合酶或粗提取物中的其他因子进行抑制的实验将确定 FbP 作用的目标。 这种相互作用的遗传分析将确定涉及 FbP 的调节回路发挥作用的生长条件。 %%% 该项目提供了一个了解 rRNA 合成与碳代谢耦合的分子回路的机会，这是一个对每个生物体都至关重要的问题，但尚未在任何实验系统中得到阐明。