Regulation and function of yeast transcriptional activator ADR1

本研究的目的是阐明 真核基因调控选择进行研究的系统是 酵母转录激活因子ADR1对葡萄糖的调控 抑制性醇脱氢酶（ADH 2）。 将特别强调 将重点放在定义ADR1的调节机制上， ADr1的功能区域，介导或 影响ADr1功能，以及这些因素和 ADR1控制ADH 2的转录。 一种生物化学， 将使用重组DNA和遗传分析。 的 ADR1的激活/抑制结构域将被精确鉴定 并分析它们之间的相互作用。 一种机制， ADR1突变增强ADR1激活结构域的功能， 通过测定其对二聚化、ADR 1 构象和其他蛋白质的结合。 蛋白质， 将与ADR 1的共免疫沉淀物纯化并表征。 %%%% 这项研究的目的是了解细胞如何对 环境和控制何时以及哪些基因的表达将 以确定其重要区域为特征。 这些 区域包括响应于环境信号的段， 与其他蛋白质接触，开启或关闭 基因. 基因、重组DNA和 将使用生物化学技术。

The goal of this research is to elucidate the mechanisms of eucaryotic gene regulation. The system chosen for study is the yeast transcriptional activator ADR1 in its control of glucose repressible alcohol dehydrogenase (ADH2). Particular emphasis will be placed on defining the mechanisms by which ADR1 is regulated, the functional regions of ADr1, the factors which mediate or influence ADr1 functions, and the means by which these factors and ADR1 control ADH2 transcription. A combination of biochemical, recombinant DNA, and genetic analyses will be used. The activation/repression domains of ADR1 will be precisely identified and their interactions will be analyzed. The mechanism by which an ADR1 mutation enhances the function of ADR1 activation domains will be examined by determining its effect on dimerization, ADR1 conformation, and binding of other proteins. The proteins which co-immuneprecipitate with ADR1 will be purified and characterized. %%%% The goal of this research is to understand how cells respond to the environment and control when and which genes the expression will be characterized by identifying their important regions. These regions include segments that respond to the environmental signals, that contact other proteins, and that turn on or off the express of genes. A combination of genetic, recombinant DNA, and biochemical techniques will be used.