Regulation and function of yeast transcriptional activator ADR1

酵母转录激活因子ADR1的调控和功能

• 批准号: 9218728
• 负责人: Clyde Denis
• 金额: $ 26.6万
• 依托单位: University of New Hampshire
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-04-01 至 1997-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9218728&HistoricalAwards=false
• 关键词: Regulation; function; yeast; transcriptional; activator

The goal of this research is to elucidate the mechanisms of eucaryotic gene regulation. The system chosen for study is the yeast transcriptional activator ADR1 in its control of glucose repressible alcohol dehydrogenase (ADH2). Particular emphasis will be placed on defining the mechanisms by which ADR1 is regulated, the functional regions of ADr1, the factors which mediate or influence ADr1 functions, and the means by which these factors and ADR1 control ADH2 transcription. A combination of biochemical, recombinant DNA, and genetic analyses will be used. The activation/repression domains of ADR1 will be precisely identified and their interactions will be analyzed. The mechanism by which an ADR1 mutation enhances the function of ADR1 activation domains will be examined by determining its effect on dimerization, ADR1 conformation, and binding of other proteins. The proteins which co-immuneprecipitate with ADR1 will be purified and characterized. %%%% The goal of this research is to understand how cells respond to the environment and control when and which genes the expression will be characterized by identifying their important regions. These regions include segments that respond to the environmental signals, that contact other proteins, and that turn on or off the express of genes. A combination of genetic, recombinant DNA, and biochemical techniques will be used.

本研究的目的是阐明真核生物基因调控的机制。本研究选用酵母转录激活剂ADR1调控葡萄糖抑制醇脱氢酶（ADH2）。重点将放在定义ADR1被调节的机制，ADR1的功能区域，介导或影响ADR1功能的因素，以及这些因素和ADR1控制ADH2转录的方法。将使用生物化学、重组DNA和遗传分析相结合的方法。ADR1的激活/抑制结构域将被精确识别，它们之间的相互作用将被分析。ADR1突变增强ADR1激活域功能的机制将通过确定其对二聚化、ADR1构象和其他蛋白质结合的影响来研究。对与ADR1共免疫沉淀的蛋白进行纯化和鉴定。%%%%这项研究的目标是了解细胞如何对环境做出反应，并通过识别其重要区域来控制何时以及哪些基因的表达。这些区域包括对环境信号作出反应的片段，与其他蛋白质接触的片段，以及开启或关闭基因表达的片段。将使用基因、重组DNA和生化技术的结合。