A Molecular Imager for Studies in Signal Transduction

用于信号转导研究的分子成像仪

• 批准号: 9970176
• 负责人: Clyde Denis
• 金额: $ 6.86万
• 依托单位: University of New Hampshire
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-10-01 至 2000-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9970176&HistoricalAwards=false
• 关键词: Molecular; Imager; Studies; Signal; Transduction

Project AbstractDBI-9970176A Molecular Imager will be used for quantification of radioistopes, fluorescence and chemiluminescence signals in studies of signal transduction. The seven major users are junior and senior faculty, funded by national agencies. Studies encompass the types of signals involved, initial perception, the signal cascade, and mechanisms by which downstream transcription is regulated in bacteria, plants and animals. These studies require quantitative high-resolution imaging for the analysis of enzymatic activities, binding studies, the identification and quantification of signaling complex proteins, and quantification of RNA and protein expression levels. Undergraduate students, graduate students and postdoctoral fellows will be trained on the Imager. The Imager will be used in two upper level undergraduate laboratory courses. Understanding the complex biological pathway of signal transduction will increase knowledge of bacterial, plant and animal developmental and regulatory pathways.

DBI-9970176 A分子成像仪将用于信号转导研究中放射性同位素、荧光和化学发光信号的定量。 七个主要用户是初级和高级教师，由国家机构资助。 研究包括所涉及的信号类型，初始感知，信号级联，以及细菌，植物和动物中下游转录调节的机制。这些研究需要定量高分辨率成像来分析酶活性、结合研究、信号复合物蛋白的鉴定和定量以及RNA和蛋白质表达水平的定量。本科生、研究生和博士后研究员将接受成像仪的培训。该成像仪将用于两个较高水平的本科实验室课程。 了解复杂的生物信号转导途径将增加细菌，植物和动物的发育和调节途径的知识。