Sulfide Detoxification Mechanisms of the Epithelia of Urechis Caupo

Urechis Caupo 上皮细胞的硫化物解毒机制

• 批准号: 9218732
• 负责人: Alissa Arp
• 金额: $ 28.93万
• 依托单位: San Francisco State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-03-01 至 1997-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9218732&HistoricalAwards=false
• 关键词: Sulfide; Detoxification; Mechanisms; Epithelia; Urechis

Hydrogen sulfide is an extremely toxic industrial waste responsible for a number of deaths in California in the last year alone. These incidents involved a paper recycling factory in Los Angeles enveloped by a cloud of toxic sulfide gas and a railroad spill of metam sodium resulting in the release of methyl isothiocyanate and hydrogen sulfide gas as breakdown products. The proposed project investigates sulfide detoxification in the peripheral tissues of a marine worm. Although our previous studies of adaptations to low oxygen and sulfide indicate that this animal detoxifies sulfide in internal fluids, the question of how the body tissues are protected or tolerate toxic sulfide remains unanswered. Preliminary data suggest that the peripheral tissues possess distinct sulfide detoxification or tolerance mechanisms, including the presence of sulfide-oxidizing bodies and symbiotic bacteria. The binding or transformation of sulfide to nontoxic forms of sulfur in the external body tissues. Elucidation of these peripheral defense mechanisms will provide a significant contribution to the understanding of tolerance and treatment of sulfide poisoning. Additionally, the studies outlined in the proposal will contribute to the well established field of sulfide metabolism and detoxification in animals. Funding of the research project would result in continued assistance to undergraduate and graduate students and be a significant contribution to the research environment at San Francisco State University.

硫化氢是一种剧毒的工业废物，仅去年一年就导致加州多人死亡。这些事件涉及洛杉矶的一家纸张回收厂被有毒硫化物气体云包围，以及一起铁路泄漏事件，导致异硫氰酸甲酯和硫化氢气体作为故障产物泄漏。这项拟议的项目研究海洋蠕虫周围组织中的硫化物解毒作用。尽管我们之前对低氧和硫化物的适应研究表明，这种动物可以使体内液体中的硫化物解毒，但如何保护或耐受有毒的硫化物的问题仍然没有答案。初步数据表明，周围组织具有明显的硫化物解毒或耐受机制，包括硫化物氧化体和共生菌的存在。硫化物在体外组织中将硫化物结合或转化为无毒形式的硫。阐明这些外周防御机制将为理解硫化物中毒的耐受和治疗提供重要的帮助。此外，提案中概述的研究将有助于在动物中建立硫化物新陈代谢和解毒领域。对研究项目的资助将导致对本科生和研究生的持续援助，并对旧金山州立大学的研究环境做出重大贡献。