Biochemistry of 2,4,5-trichlorophenoxyacetic acid degradation

虽然多卤代芳香族化合物是主要的环境污染物，但对这些化合物的生物降解或解毒所涉及的酶知之甚少。我们建议使用2，4，5-三氯苯氧乙酸（2，4，5-T）的降解假单胞菌洋葱AC 1100作为多卤代芳香族化合物的生物降解模型。我们建议识别，纯化和表征参与2，4，5-T降解的酶。在其中一种酶被纯化后，将确定部分氨基酸序列。最后，蛋白质的结构基因将被克隆使用DNA探针构建与酶的氨基酸序列数据，并进行了表征。我们的工作结果和Chakrabarty小组关于2，4，5-T降解的其他遗传信息将共同为我们了解多卤代芳香族化合物的微生物降解做出重要贡献。多卤代芳香族化合物是主要的环境污染物;然而，对这些化合物的微生物降解的生物化学知之甚少。为了使用生物方法从我们的环境中去除这些化合物，有必要了解这些化合物的微生物降解的生物化学。以洋葱假单胞菌降解2，4，5-三氯苯氧乙酸（2，4，5-T）为模型系统，研究微生物降解多卤代芳香族化合物的生物化学。我们将鉴定和分离参与2，4，5-T降解的酶，并克隆编码这些酶的相应基因。本研究结果将为多卤代芳香族化合物的微生物降解提供基础资料。

Although polyhalogenated aromatic compounds are major environmental pollutants, little is known about the enzymes involved in the biodegradation or detoxification of these compounds. We propose to use the degradation of 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) by Pseudomonas cepacia AC1100 as a model for the biodegradation of polyhalogenated aromatic compounds. We propose to identify, purify, and characterize enzymes involved in 2,4,5-T degradation. After one of the enzymes is purified, a partial amino acid sequence will be determined. Finally, the structural gene for the protein will be cloned using DNA probes constructed with amino acid sequence data about the enzyme, and characterized. The results from our work and additional genetic information about 2,4,5-T degradation from Chakrabarty's group will together form an important contribution to our understanding about microbial degradation of polyhalogenated aromatic compounds. %%% Polyhalogenated aromatic compounds are major environmental pollutants; however, little is known about the biochemistry of microbial degradation of such compounds. In order to use biological approaches to remove these compounds from our environment, it is necessary to understand the biochemistry of microbial degradation of these compounds. We proposed to use the degradation of 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) by Pseudomonas cepacia as a model system to study the biochemistry of microbial degradation of polyhalogenated aromatic compounds. We will identify and isolate enzymes involved in 2,4,5-T degradation and clone the corresponding genes encoding the enzymes. The results from this work will provide fundamental information on microbial degradation of polyhalogenated aromatic compounds.