An LC/MS Instrument for Biological Research

生物研究的LC/MS仪器LC/MS(LC/MS)仪器特别适用于检测和鉴定具有生物意义的小有机化合物，如代谢中间体、有机激素、抗生素和天然有机化合物。该仪器还可以确定蛋白质的相对分子质量，并可用于评估翻译后修饰。几个研究领域将从这一工具中受益。受益最大的领域是环境污染物的生物降解研究。通过检测和鉴定代谢中间体，LC/MS将有助于阐明有机污染物的生物降解途径。污染物很多，了解它们是如何降解的很重要，这样才能设计出有效的生物修复方法。另一个将立即受益的研究领域是植物生物化学。LC/MS将有助于研究具有较高经济价值的植物有机化合物的生物合成途径、植物细胞中的蛋白质生产以及植物/微生物共生体的固氮作用。这项奖励将允许华盛顿州立大学购买Waters LC/MS仪器。LC组件是任何高性能LC仪器的基本组件，建议使用Waters公司(Waters Corp.，Milford，MA)的2690XE分离模块，该模块由两个泵和一个自动进样器组成。MS检测器建议为Waters的ZMD[4000]检测器。该探测器工作在电喷雾模式或大气压化学电离模式下，检测质量范围从50到4000道尔顿。LC/MS仪器是生物研究人员必不可少的仪器。这项奖励允许华盛顿州立大学理学院和工程与建筑学院为该学院购买第一台LC/MS。预计将有大约17名本科生、24名研究生和5名博士后助理接受使用LC/MS的培训。此外，参加华盛顿州立大学培训项目的学生将使用该仪器，包括参加多相环境研究中心NSF IGERT项目的学生。

ABSTRACT0070398Luying XunWashington State UniversityAn LC/MS Instrumentation for Biological ResearchA liquid chromatography/mass spectrometry (LC/MS) instrument is especially useful for the detection and identification of small organic compounds of biological interests, such as metabolic intermediates, organic hormones, antibiotics, and natural organic compounds. The instrument can also determine the molecular weights of proteins and can be used to assess post-translational modifications. Several research areas will benefit from this instrument. The area that will benefit the most is the study of biodegradation of environmental pollutants. The LC/MS will help elucidate biodegradation pathways of organic pollutants by detecting and identifying the metabolic intermediates. There are many pollutants and it is important to know how they are degraded so that effective methods can be designed for bioremediation. Another research area that will immediately benefit is plant biochemistry. The LC/MS will help in the areas of biosynthetic pathways of plant organic compounds that have high economic values, protein production in plant cells, and nitrogen fixation by plant/microorganism symbionts. This award will allow Washington State University to purchase a Waters LC/MS instrument. The LC component, which is the basic component in any high-performance LC instrument, is proposed to be the Waters' (Waters Corp., Milford, MA) 2690XE separation module consisting of two pumps and an autosampler. The MS detector is proposed to be Waters' ZMD [4000] detector. This detector operates in either electrospray mode or atmospheric pressure chemical ionization mode and detects a mass range from 50 to 4,000 Daltons.LC/MS instruments are essential for biological researchers. This award allows the College of Science and the College of Engineering and Architecture at Washington State University to purchase the first LC/MS for the college. It is expected that approximately 17 undergraduate students, 24 graduate students, and 5 postdoctoral associates will be trained to use LC/MS. Moreover, the instrument will be used by students participating in training programs at Washington State University, including those students participating in the NSF IGERT program in the Center for Multiphase Environmental Research.