Molecular Analysis of Chlamydomonas Mating-Type Locus

衣藻交配型基因座的分子分析

• 批准号: 9218817
• 负责人: Ursula Goodenough
• 金额: $ 31.5万
• 依托单位: Washington University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-03-01 至 1997-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9218817&HistoricalAwards=false
• 关键词: Molecular; Analysis; Chlamydomonas; Mating; Type

The unicellular green alga Chlamydomonas reinhardtii differentiates into 4 distinct cell types during the course of its life cycle. Most if not all of these differentiation programs are governed by its mating-type (mt) locus which exists as a pair of apparent alleles (mt+ and mt-). A chromosome walk has documented that the mt loci include highly rearranged sectors which render mt+ and mt- very dissimilar to one another. By analogy with fungal mating-type loci this rearranged domain may include genes that govern life-cycle transitions. One such gene has already been identified in the region: the imp-1 gene, which confers upon mt+ gametes the ability to fuse with mt- gametes, has been localized to a segment of the rearranged domain by virtue of its disruption in imp-1 mutants. Additional sequences from the rearranged domains will be used to identify additional mt-linked genes, to identify cDNA clones derived from cell-type-specific, mt-linked genes, and to identify genes whose products regulate the mating phenotype of gametic cells. %%% This analysis should enhance our understanding of the evolution of eukaryotic gene regulation and cell differentiation.

单细胞绿色衣藻 分化成4种不同的细胞类型在其过程中， 生命周期 大多数（如果不是所有）这些差异化计划都是 由其交配型（mt）基因座控制， 表观等位基因（mt+和mt-）。 染色体漫步记录了 MT基因座包括高度重排的区段， 和mt-彼此非常不同。 与真菌相似 交配型基因座这种重排的结构域可能包括基因， 管理生命周期过渡。 一个这样的基因已经被 在该地区确定：imp-1基因，赋予MT+ 配子与mt配子融合的能力，已经被定位为 重排结构域的一部分，由于它的破坏， imp-1突变体。 来自重排结构域的额外序列 将被用来识别其他的线粒体连锁基因， 来自细胞类型特异性、mt连锁基因的cDNA克隆，以及 确定其产物调节交配表型的基因， 配子细胞 %%% 这一分析应有助于我们了解 真核基因调控和细胞分化。