Neural Control of Proteolysis in Specific Muscle Cells

特定肌肉细胞中蛋白水解的神经控制

• 批准号: 9218839
• 负责人: Lewis Jacobson
• 金额: $ 22.5万
• 依托单位: University of Pittsburgh
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-06-01 至 1996-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9218839&HistoricalAwards=false
• 关键词: Neural; Control; Proteolysis; Specific; Muscle

The purpose of this project is to determine how signals from nerve cells control the degradation of cellular proteins in muscle. The experimental subject is a small roundworm, Caenorhabditis elegans, that has a relatively simple system of nerves and muscles. This organism is also conducive to simple and rapid genetic experiments. To observe protein degradation, we have introduced a foreign gene from a bacterium (E. coli) into the worm so that the fate of this protein can be easily followed by biochemical and immunochemical methods. Our objective is to use mutants that affect the function of nerves or muscles to determine how direct nerve-to-muscle connections and/or chemical signaling between nerves and muscles affects the rate of protein breakdown inside muscle cells.

该项目的目的是确定神经细胞的信号如何控制肌肉中细胞蛋白质的降解。实验对象是一种小型蛔虫，即秀丽隐杆线虫，它具有相对简单的神经和肌肉系统。这种生物体也有利于简单快速的基因实验。为了观察蛋白质降解，我们将来自细菌（大肠杆菌）的外源基因引入蠕虫中，以便可以通过生化和免疫化学方法轻松跟踪该蛋白质的命运。我们的目标是利用影响神经或肌肉功能的突变体来确定神经与肌肉的直接连接和/或神经与肌肉之间的化学信号传导如何影响肌肉细胞内蛋白质分解的速率。