Neural Control of Proteolysis in Specific Muscle Cells

这个项目的目的是确定信号如何从 神经细胞控制细胞蛋白质的降解， 肌肉.实验对象是一条小蛔虫， 秀丽隐杆线虫有一个相对简单的 神经和肌肉。这种有机体也有利于简单和 快速基因实验为了观察蛋白质降解，我们 已经从细菌中引入了外源基因（E.大肠杆菌） 这样就可以很容易地追踪这种蛋白质的命运 通过生物化学和免疫化学方法。我们的目标是 使用影响神经或肌肉功能的突变体， 确定如何直接神经肌肉连接和/或化学 神经和肌肉之间的信号传导会影响蛋白质 在肌肉细胞内分解

The purpose of this project is to determine how signals from nerve cells control the degradation of cellular proteins in muscle. The experimental subject is a small roundworm, Caenorhabditis elegans, that has a relatively simple system of nerves and muscles. This organism is also conducive to simple and rapid genetic experiments. To observe protein degradation, we have introduced a foreign gene from a bacterium (E. coli) into the worm so that the fate of this protein can be easily followed by biochemical and immunochemical methods. Our objective is to use mutants that affect the function of nerves or muscles to determine how direct nerve-to-muscle connections and/or chemical signaling between nerves and muscles affects the rate of protein breakdown inside muscle cells.