In vitro mutagenesis of a Bacteriophge self-splicing Intron

噬菌体自剪接内含子的体外诱变

• 批准号: 9219401
• 负责人: Ann Zeeh
• 金额: $ 11.65万
• 依托单位: College of Saint Rose
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-02-01 至 1997-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9219401&HistoricalAwards=false
• 关键词: vitro; mutagenesis; Bacteriophge; self; splicing

According to the central dogma of molecular biology, the information contained in the hereditary material, DNA, is passed to proteins via an intermediate molecule called RAN. Proteins direct the control of most cell functions. While the overall process of gene expression is understood, the details of the mechanisms involved in gene expression need clarification. For example, intervening sequences (or introns) are extra pieces of information inside genes that are not used to produce the final gene product. The significance of introns inside genes remains to be determined. This project is looking at requirements for splicing , in the bacterial virus T4, a process that removes introns from RNA during gene expression. RNA splicing mechanisms are varied, but in all cases, the sequence of nucleotides (chemical subunits of RNA and DNA) is important to the splicing process. %%% In this project, changes in the nucleotide sequence of the introns will be generated by chemical mutagenesis and the effect of these changes on splicing and gene expression will be evaluated. The overall goal of this study is to use chemically induced changes in the nucleotide sequences of introns to identify particular nucleotides in the introns important to splicing. This information will be used to enhance models for splicing mechanisms.

根据分子生物学的中心法则， 遗传物质DNA中的信息被传递给 蛋白质通过中间分子RAN。 蛋白质直接 控制大多数细胞功能。 虽然整个过程 了解基因表达、机制细节 基因表达需要澄清。 比如说， 插入序列（或内含子）是额外的信息片段， 在基因内部，这些基因不用于产生最终的基因产物。 基因内内含子的重要性仍有待确定。 该项目正在研究拼接的要求，在 细菌病毒T4，一个过程，从RNA中删除内含子， 基因表达。 RNA剪接机制多种多样，但在所有 在某些情况下，核苷酸序列（RNA的化学亚基和 DNA）对剪接过程很重要。 %%% 在这个项目中，内含子的核苷酸序列的变化 将通过化学诱变产生， 将评估剪接和基因表达的变化。 的 这项研究的总体目标是利用化学诱导的变化， 内含子的核苷酸序列，以确定特定的 内含子中对剪接很重要的核苷酸。 这些信息 将用于增强拼接机制的模型。