Cytochrome P450 Forms, Function and Regulation in Antarctic Fish Species

将对一些南极鱼类物种的细胞色素P450蛋白的结构和功能特性及其调节进行研究。这些蛋白质在许多激素以及污染物和致癌物的新陈代谢和生物学作用中起着至关重要的作用。其中一些蛋白质是由污染化学物质诱导的，是接触的理想标志。几种P450蛋白的基因，以及基因产物将被研究。将描述由碳氢化合物或多氯联苯诱导的P450以及参与该基因调控的受体。目的是确定膜结合酶在低温冷适应动物中的结构和功能适应性，它们的基因调控和受体机制。其他目标是为评估外来化学物质对南极鱼类可能起作用的程度提供分子和机制基础。这些研究应提供许多物种的最终基线和档案样本，以便与未来样本和分析的结果进行比较。这一结果将对探测和跟踪南大洋化学品目前和未来的影响产生影响，并可能区分来自全球和/或当地南极的化学品。

The structural and functional properties of cytochrome P450 proteins and their regulation will be examined for a number of Antarctic fish species. These proteins are critical in the metabolism and biological action of many hormones, as well as pollutants and carcinogens. Some of these proteins are induced by pollutant chemicals and are ideal markers for exposure. The genes for several P450 proteins, and the gene products will be studied. The P450 that is induced by hydrocarbons or polychlorinated biphenyls and the receptors involved in the regulation of the gene will be characterized. The objectives are to identify structural and functional adaptions of membrane bound enzymes, their gene regulation and receptor mechanisms in stenothermal cold-adapted animals. Additional objectives are to provide a molecular and mechanistic basis for evaluating the degree to which foreign chemicals may be acting in Antarctic fish. These studies should provide a definitive baseline and archival samples of numerous species, against which results of future samples and analyses can be compared. The results will have implications for detecting and following the present and future effects of chemicals in the Southern Ocean and may distinguish between chemicals with global and/or local Antarctic sources.