Cytochrome P450 Forms, Function and Regulation in Antarctic Fish Species

南极鱼类细胞色素 P450 的形式、功能和调节

• 批准号: 9219418
• 负责人: John Stegeman
• 金额: $ 14.96万
• 依托单位: Woods Hole Oceanographic Institution
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-02-15 至 1995-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9219418&HistoricalAwards=false
• 关键词: Cytochrome; P450; Forms; Function; Regulation

The structural and functional properties of cytochrome P450 proteins and their regulation will be examined for a number of Antarctic fish species. These proteins are critical in the metabolism and biological action of many hormones, as well as pollutants and carcinogens. Some of these proteins are induced by pollutant chemicals and are ideal markers for exposure. The genes for several P450 proteins, and the gene products will be studied. The P450 that is induced by hydrocarbons or polychlorinated biphenyls and the receptors involved in the regulation of the gene will be characterized. The objectives are to identify structural and functional adaptions of membrane bound enzymes, their gene regulation and receptor mechanisms in stenothermal cold-adapted animals. Additional objectives are to provide a molecular and mechanistic basis for evaluating the degree to which foreign chemicals may be acting in Antarctic fish. These studies should provide a definitive baseline and archival samples of numerous species, against which results of future samples and analyses can be compared. The results will have implications for detecting and following the present and future effects of chemicals in the Southern Ocean and may distinguish between chemicals with global and/or local Antarctic sources.

细胞色素P450的结构和功能特性 蛋白质及其调节将被检查的一些 南极鱼类这些蛋白质在 许多激素的代谢和生物作用，以及 污染物和致癌物。其中一些蛋白质是由 污染化学品，是理想的暴露标记。的基因 几种P450蛋白，并将研究基因产物。 由碳氢化合物或多氯化合物诱导的P450 联苯和参与基因调控的受体 将被定性。目标是确定结构 以及膜结合酶及其基因的功能适应 狭温冷适应的调控和受体机制 动物另外的目的是提供一种分子和 评估外国投资者在多大程度上 化学物质可能对南极鱼类起作用。这些研究应 提供了一个明确的基线和档案样本的许多 物种，未来的样本和分析结果可以 被比较。这些结果将对检测和 化学品目前和未来的影响， 南大洋和可能区分化学品与全球 和/或当地南极来源。