U.S. GLOBEC: Analysis of Short-Term Growth in Copepods and Larval Fish Using Molecular Markers of Cell Proliferation

将微观和宏观的物理因素与类鱿鱼类桡足类、鳕鱼和黑线鳕幼虫的生长联系起来，对美国- globec乔治银行项目至关重要。先前测量生长的方法包括形态计量学、生命阶段持续时间、产蛋量和间接生化方法，如RNA/DNA比率。Stegeman博士将利用免疫组织化学、组织学和荧光学方法来检测细胞增殖，并继续将这项技术应用于海洋鱼类和无脊椎动物。该方法使用合成细胞核DNA的细胞分子标记的单克隆抗体来对这些细胞进行定量。早期的目标将是确定哪种细胞和器官的增殖率最能反映总体的生长速度，这是用形态计量学方法在鱼类幼体中测量的，用阶段长度在桡足动物中测量的。测量这种差异的时间尺度将在一系列实验室实验中进行研究。随后，他们将参加正在计划中的US-GLOBEC过程巡航，以开发和在海上应用该方法。参加这项研究将把由于短期和长期气候变化而波动的垂直混合和分层的物理测量转化为对这些参数的生物效应的即时分析。＊＊＊

9313676 Stegeman Connecting micro- and broad-scale physical factors to growth in calanoid copepods and larval cod and haddock is essential to the US-GLOBEC Georges Bank Program. Prior methods to measure growth have involved morphometrics, life stage durations, egg production, and indirect biochemical methods, such as the RNA/DNA ratio. Dr. Stegeman will utilize immunohistochemical, histological and fluorometric methods to detect cell proliferation, continuing the application of this technique in marine fish and invertebrates. The method uses monoclonal antibodies to molecular markers of cells synthesizing nuclear DNA to allow quanitification of such cells. An early aim will be to establish which cell and organ proliferation rates most closely reflect overall growth rate, as measured morphometrically in larval fish, and by stage length in copepods. The time scale over which such differences can be measured will be studied in a series of laboratory experiments. Subsequently, they will participate in the US-GLOBEC Process cruises that are being planned, to develop and apply the method at sea. Participation in this study will translate physical measurements of vertical mixing and stratification, that fluctuate as a result of short and long term climatic changes, into an instant assay for the biological effects of such parameters. ***