U.S. GLOBEC: Analysis of Short-Term Growth in Copepods and Larval Fish Using Molecular Markers of Cell Proliferation
美国 GLOBEC:利用细胞增殖的分子标记分析桡足类和幼鱼的短期生长
基本信息
- 批准号:9313676
- 负责人:
- 金额:$ 33.94万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:1993
- 资助国家:美国
- 起止时间:1993-09-01 至 1997-02-28
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
9313676 Stegeman Connecting micro- and broad-scale physical factors to growth in calanoid copepods and larval cod and haddock is essential to the US-GLOBEC Georges Bank Program. Prior methods to measure growth have involved morphometrics, life stage durations, egg production, and indirect biochemical methods, such as the RNA/DNA ratio. Dr. Stegeman will utilize immunohistochemical, histological and fluorometric methods to detect cell proliferation, continuing the application of this technique in marine fish and invertebrates. The method uses monoclonal antibodies to molecular markers of cells synthesizing nuclear DNA to allow quanitification of such cells. An early aim will be to establish which cell and organ proliferation rates most closely reflect overall growth rate, as measured morphometrically in larval fish, and by stage length in copepods. The time scale over which such differences can be measured will be studied in a series of laboratory experiments. Subsequently, they will participate in the US-GLOBEC Process cruises that are being planned, to develop and apply the method at sea. Participation in this study will translate physical measurements of vertical mixing and stratification, that fluctuate as a result of short and long term climatic changes, into an instant assay for the biological effects of such parameters. ***
将微观和宏观的物理因素与类鱿鱼类桡足类、鳕鱼和黑线鳕幼虫的生长联系起来,对美国- globec乔治银行项目至关重要。先前测量生长的方法包括形态计量学、生命阶段持续时间、产蛋量和间接生化方法,如RNA/DNA比率。Stegeman博士将利用免疫组织化学、组织学和荧光学方法来检测细胞增殖,并继续将这项技术应用于海洋鱼类和无脊椎动物。该方法使用合成细胞核DNA的细胞分子标记的单克隆抗体来对这些细胞进行定量。早期的目标将是确定哪种细胞和器官的增殖率最能反映总体的生长速度,这是用形态计量学方法在鱼类幼体中测量的,用阶段长度在桡足动物中测量的。测量这种差异的时间尺度将在一系列实验室实验中进行研究。随后,他们将参加正在计划中的US-GLOBEC过程巡航,以开发和在海上应用该方法。参加这项研究将把由于短期和长期气候变化而波动的垂直混合和分层的物理测量转化为对这些参数的生物效应的即时分析。***
项目成果
期刊论文数量(0)
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科研奖励数量(0)
会议论文数量(0)
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John Stegeman其他文献
Tracking sea trout, cod, wrasse, salmon and sharks around Bergen
追踪卑尔根周围的海鳟鱼、鳕鱼、濑鱼、鲑鱼和鲨鱼
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
K. Vollset;Saron Behre;Anne Christine Knag;Lotte S. Dahlmo;Robert J. Lennox;T. Thorsnes;L. Bjarnadóttir;Hanne Hodnesdal;Frithjof Moy;Yalei Li;K. Hancke;S. Basedow;Kanchana Bandara;Cait L. McCarry;S. Falk‐Petersen;Lionel Camus;David McKee;Huizi Dong;Meng Zhou;Emlyn Davies;Walker O. Smith;E. Vereide;Hernes;Mihaljevic;Browman;Fields;M. D. Agersted;Titelman;de Jong;A. F. Opdal;Peter Wright;Geir Blom;H. Höffle;Olav S. Kjesbu;Marta Moyano;Anna Akimova;G. Börner;Cindy van Damme;Romain Frelat;M. Peck;A. Gro;Vea Salvanes;Natalya Gallo;A. Folkvord;Arved Staby;M. R. Solås;F. Saltalamacchia;Dag L Aksnes;S. Kaartvedt;Lisa Levin;Karin Limburg;F. Midtøy;H. Savolainen;Carl Bukowski;Øivind Andersen;Karen Rosland;Anders Goksøyr;G. Alendal;Augustine Arukwe;M. Celander;D. Dankel;Nancy Denslow;M. Eide;S. Goksøyr;B. E. Grøsvik;K. Hylland;Inge Jonassen;J. Lyche;M. Müller;P. Olsvik;D. Pampanin;Cinta Porte;John Stegeman;F. Yadetie;Odd;André Karlsen;Gaute W. Seljestad;Mar´ıa Quintela;Ellika Faust;K. Halvorsen;F. Besnier;E. Jansson;K. A. Glover;Andreas Altenburger;Huimin Cai;Qiye Li;K. Drumm;Miran Kim;Yuanzhen Zhu;L. Garcia‐Cuetos;Xiaoyu Zhan;P. J. Hansen;Uwe John;Shuaicheng Li;N. Lundholm - 通讯作者:
N. Lundholm
Reciprocal cross-talk between Ahr2 and Pxr signaling in response to PCB126 and pregnenolone in developing zebrafish
斑马鱼发育过程中 Ahr2 和 Pxr 信号对 PCB126 和孕烯醇酮的相互串扰
- DOI:
- 发表时间:
2016 - 期刊:
- 影响因子:0
- 作者:
Akira Kubota;Jared Goldstone;John Stegeman - 通讯作者:
John Stegeman
John Stegeman的其他文献
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{{ truncateString('John Stegeman', 18)}}的其他基金
Woods Hole Center for Oceans and Human Health
伍兹霍尔海洋与人类健康中心
- 批准号:
1840381 - 财政年份:2018
- 资助金额:
$ 33.94万 - 项目类别:
Continuing Grant
WHCOHH: Harmful algal bloom dynamics and epigenetic mechanism of toxin action
WHCOHH:有害藻华动态和毒素作用的表观遗传机制
- 批准号:
1314642 - 财政年份:2013
- 资助金额:
$ 33.94万 - 项目类别:
Continuing Grant
The Woods Hole Center for Oceans and Human Health
伍兹霍尔海洋与人类健康中心
- 批准号:
0911031 - 财政年份:2009
- 资助金额:
$ 33.94万 - 项目类别:
Continuing Grant
2008 Oceans and Human Health GRC-Sponsored Conference & Graduate Research Seminar
2008 年 GRC 主办的海洋与人类健康会议
- 批准号:
0801434 - 财政年份:2008
- 资助金额:
$ 33.94万 - 项目类别:
Standard Grant
The Woods Hole Center for Oceans and Human Health
伍兹霍尔海洋与人类健康中心
- 批准号:
0430724 - 财政年份:2004
- 资助金额:
$ 33.94万 - 项目类别:
Continuing Grant
Cytochrome P450 Forms, Function and Regulation in Antarctic Fish Species
南极鱼类细胞色素 P450 的形式、功能和调节
- 批准号:
9219418 - 财政年份:1993
- 资助金额:
$ 33.94万 - 项目类别:
Standard Grant
Marine Biotechnology Instrumentation for Woods Hole Oceanographic Institution and the Marine Biological Laboratory
伍兹霍尔海洋研究所和海洋生物实验室的海洋生物技术仪器
- 批准号:
8812656 - 财政年份:1989
- 资助金额:
$ 33.94万 - 项目类别:
Standard Grant
Chemicals and Reproductive Systems in Marine Animals: Cytochromes P-450 and Biotransformation of Xenobiotics and Steroids in Fish
海洋动物的化学品和生殖系统:细胞色素 P-450 以及鱼类中异生物质和类固醇的生物转化
- 批准号:
8310505 - 财政年份:1983
- 资助金额:
$ 33.94万 - 项目类别:
Continuing Grant
Pollutant Responses in Marine Animals: Cytochrome P-450 And Biotransformation of Hydrocarbons in Marine Fish
海洋动物的污染物反应:细胞色素 P-450 和海洋鱼类中碳氢化合物的生物转化
- 批准号:
8018569 - 财政年份:1981
- 资助金额:
$ 33.94万 - 项目类别:
Continuing Grant
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