Advanced IMAC Methodology

固定化金属离子亲和层析（IMAC）已被证明 可用于分离微量存在的蛋白质， 复杂的混合物 该奖项的目的是描述 并映射几个IMA吸附剂，并使用这些结果 研究制定和测试方案， 使用级联模式多亲和性从粗混合物中分离蛋白质 色谱法（CASMAC）。 第一个目标是描述新的 凝胶对于螯合剂、金属离子和 色谱条件 在其他方法中，吸附 将使用等温线和NMR。 其次，这些凝胶将 使用氨基酸和模型肽的保留研究作图 和蛋白质。 第三，调查人员计划制定和 允许复杂蛋白质分级分离测试方案 使用CASMAC的混合物。 预计这项工作将 使吸附剂可供科学界使用 对于IMAC和CASMAC，它们提供了 蛋白质和肽，并专门针对 与不同氨基酸侧基的相互作用。 进一步 研究人员计划使用一系列 分离复杂蛋白质混合物的亲和技术 有效地

Immobilized metal ion affinity chromatography (IMAC) has proved useful in isolating proteins present in trace amounts from complex mixtures. The objective of this award is to characterize and map several IMA-adsorbents and to use the results of these studies to formulate and test protocols for the isolation of proteins from crude mixtures using cascade mode multi-affinity chromatography (CASMAC). The first goal is to characterize new gels with regard to chelating agents, metal ions and chromatography conditions. Among other methods, adsorption isotherms and NMR will be used. Secondly, these gels will be mapped using retention studies of amino acids, and model peptides and proteins. Thirdly, the investigators plan to develop and test protocols that allow fractionation of complex protein mixtures using CASMAC. It is anticipated that this work will result in making adsorbents available to the scientific community for both IMAC and CASMAC that offer incremental affinities for proteins and peptides, and that are specifically targeted for interaction with different amino acid side groups. Further the investigators plan to develop a rational approach using a series of affinity techniques to separate complex protein mixtures effectively.