SGER: Separation and Purification of High Molecular Weight Homologous Blood Proteins using IMAC

这笔拨款是通过化学和运输系统部界面、运输和分离项目的分离和净化项目子项目授予的。首席研究员是UMBC的Duane Bruley博士。该提案描述了一个项目，研究一种称为固定金属亲和色谱（IMAC）的方法，用于从天然血液中提取高分子量蛋白质。它有可能产生一种比基于使用单克隆抗体和利用rDNA技术的方法更便宜的方法来做到这一点。提取的蛋白质可能对包括血友病A和深静脉血栓（DVT）在内的许多疾病的治疗具有重要意义。目前治疗这些疾病的药物有严重的副作用。PI概述了一个项目，研究他从美国红十字会提供的血浆和弗吉尼亚理工学院提供的转基因猪奶中分离蛋白C的技术。

This grant is awarded through the Separations and Purification program sub-element of the Interfacial, Transport and Separations program of the Chemical and Transport Systems Division. The principal investigator is Dr. Duane Bruley at UMBC. The proposal describes a program to study a method called Immobilized Metal Affininty Chromatography (IMAC ) for extracting high molecular weight proteins from natural blood. It has the potential for yielding a less expensive route for doing this than methods based upon the use of monoclonal antibodies as well as those exploiting rDNA technology. The proteins to be extracted could be important in the treatment of many diseases including hemphilia A and deep vein thrombosis(DVT). Current therapies for these conditions involve drugs with serious side-effects. The PI has outlined a program for studying his technique for isolating protein C from both blood plasma supplied by the American Red Cross and transgenic swine milk supplied by Virginia Polytechnic Institute.