SGER: Separation and Purification of High Molecular Weight Homologous Blood Proteins using IMAC

SGER：使用 IMAC 分离和纯化高分子量同源血液蛋白

• 批准号: 9904465
• 负责人: Duane Bruley
• 金额: $ 3万
• 依托单位: University of Maryland Baltimore County
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9904465&HistoricalAwards=false
• 关键词: SGER; Separation; Purification; Molecular; Weight

This grant is awarded through the Separations and Purification program sub-element of the Interfacial, Transport and Separations program of the Chemical and Transport Systems Division. The principal investigator is Dr. Duane Bruley at UMBC. The proposal describes a program to study a method called Immobilized Metal Affininty Chromatography (IMAC ) for extracting high molecular weight proteins from natural blood. It has the potential for yielding a less expensive route for doing this than methods based upon the use of monoclonal antibodies as well as those exploiting rDNA technology. The proteins to be extracted could be important in the treatment of many diseases including hemphilia A and deep vein thrombosis(DVT). Current therapies for these conditions involve drugs with serious side-effects. The PI has outlined a program for studying his technique for isolating protein C from both blood plasma supplied by the American Red Cross and transgenic swine milk supplied by Virginia Polytechnic Institute.

这笔赠款是通过化学和运输系统司界面、运输和分离计划的分离和净化计划子要素授予的。首席研究员是UMBC的Duane Bruley博士。该提案描述了一项研究一种名为固定化金属亲和层析(IMAC)的方法的计划，该方法用于从天然血液中提取高分子量蛋白质。与基于单抗的方法以及利用rDNA技术的方法相比，它有可能产生一种成本更低的方法来完成这项工作。所提取的蛋白质可能在包括血友病A和深静脉血栓形成(DVT)在内的许多疾病的治疗中发挥重要作用。目前针对这些疾病的治疗方法包括具有严重副作用的药物。PI概述了一项研究他的技术的计划，该技术从美国红十字会提供的血浆和弗吉尼亚理工学院提供的转基因猪奶中分离蛋白C。