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The Regulation of Leaf Cell Expansion by Light and Ion Flux

光和离子流对叶细胞扩张的调节

基本信息

批准号：
9220110
负责人：
Elizabeth Van Volkenburgh
金额：
$ 32.5万
依托单位：
University of Washington
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1993
资助国家：
美国
起止时间：
1993-05-15 至 1997-04-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9220110&HistoricalAwards=false
关键词：
Regulation Leaf Cell Expansion Light

项目摘要

Leaf growth responses to light are important for a plant to maximize its photosynthetic capacity while conserving available nutrients and water. Leaf growth is stimulated photo- morphogenically by red and blue light acting by separate mechanisms, and is enhanced by photosynthesis occurring in the growing cells. The growth response to light is associated with proton efflux, and other ion fluxes across the plasma membrane. The objectives of this study are to ask: 1. How is the plasma membrane proton pump regulated by light in epidermal and mesophyll cells? 2. Are light-induced ion fluxes in leaf cells carried in part by ion channels, and if so, does light regulate channel activity? 3. Will mutants defective in growth responses to light exhibit altered electrical responses to light? These questions will be approached by electrophysiological (microelectrode and patch clamp) techniques taking advantage of mutants of Arabidopsis showing altered growth responses to light. %%% Leaf growth responses to light are important for a plant to maximize its ability to carry out photosynthesis while conserving available nutrients and water. Leaf growth is stimulated by red and blue light and is enhanced by photosynthesis occurring in the growing cells. The growth response to light is associated with ion fluxes across the cell membrane. This project will use electrical techniques to study the mechanisms by which ions move across the membranes of leaf cells in response to light. The results of this research will clarify the initial events occurring in light responses of growing leaf cells.

叶子生长对光的反应对于植物最大限度地提高其光合作用能力同时保存可用的养分和水分非常重要。红光和蓝光通过不同的机制作用，在光形态上刺激叶子的生长，并通过生长细胞中发生的光合作用来增强叶子的生长。对光的生长响应与质子流出和穿过质膜的其他离子通量相关。本研究的目的是要问：1.表皮和叶肉细胞中的质膜质子泵是如何受光调节的？ 2. 叶细胞中光诱导的离子通量是否部分由离子通道携带？如果是，光是否调节通道活性？ 3. 对光的生长反应有缺陷的突变体是否会表现出对光的电反应改变？这些问题将通过电生理学（微电极和膜片钳）技术来解决，该技术利用显示出对光的生长反应改变的拟南芥突变体。 %%% 叶子生长对光的反应对于植物最大限度地发挥其进行光合作用的能力同时保存可用的养分和水分非常重要。叶子的生长受到红光和蓝光的刺激，并通过生长细胞中发生的光合作用而增强。对光的生长反应与穿过细胞膜的离子通量有关。该项目将使用电气技术来研究离子响应光而穿过叶细胞膜的机制。这项研究的结果将阐明生长中的叶子细胞的光反应中发生的初始事件。