U.S.-New Zealand Cooperative Research: Analysis of Coupling,A Step Required for Synthesis of the Phytotoxin Coronatine

该奖项为卡罗尔·本德博士提供旅行支持， 俄克拉荷马州州立大学进行合作研究 与罗宾·米切尔博士在山。阿尔伯特研究中心 新西兰关于P. p. dingae中冠菌素合成的研究。 的 植物毒素冠菌素是一种重要的毒力因子， 由假单胞菌引起的疾病。拟议工作 重点是偶联活性，催化酰胺键的步骤 非植物毒性前体coronafacic和 冠酰胺酸，导致冠菌素的合成。的 研究将产生一个结构特征的 偶联活性， 将尝试识别 启动子，并将鉴定调节活性的因子。 研究人员计划研究其动力学和特异性 使用无细胞测定和C14标记的偶联活性 前体，以获得冠菌素生物合成的信息。 这项研究与以下目标密切相关： 本德博士的NSF奖89-02561冠菌素的遗传学 合成。这个奖项结合了专业知识， 分子遗传学和有机生物学的两位研究者 化学，并提供本德博士暴露于放射性示踪剂 在米切尔博士的实验室工作

This award provides travel support for Dr. Carol Bender of Oklahoma State University to conduct collaborative research with Dr. Robin Mitchell at the Mt. Albert Research Center in New Zealand on coronatine synthesis in P. syringae. The phytotoxin coronatine is an important virulence factor in several diseases caused by Pseudomonas syringae. The proposed work focuses on coupling activity, a step which catalyzes amide bond formation between the nonphytotoxic precursors coronafacic and coronamic acid, resulting in the synthesis of coronatine. The research will produce a structural characterization of the coupling activity, will attempt to identify the coupling promoter, and will identify factors which regulate the activity. The investigators plan to study the kinetics and the specificity of the coupling activity using a cell-free assay and C14-labeled precursors to obtain information on coronatine biosynthesis. This research is closely related to the objectives of Dr. Bender's NSF award 89-02561 on the genetics of coronatine synthesis in P. syringae. This award combines the expertise of the two investigators in molecular genetics and organic chemistry and provides Dr. Bender exposure to the radiotracer work in Dr. Mitchell's laboratory.