RUI: Acquisition of equipment for tracking and purifying radioactive proteins

这项提议的目标是获得一个高质量的自动FPLC系统和一个新的伽马计数器，以纯化和跟踪蛋白质/多肽(特别是放射性蛋白质/多肽)及其金属或假体基团。FPLC系统是必要的，因为主要研究人员的实验室目前获得这类系统的机会非常有限，尽管在每个系统中，蛋白质/肽的纯化(用于确定其结构、功能和调节)是一项主要活动。需要一个新的伽马计数器，因为现有的伽马计数器已有12年的历史，可能会变得不可靠。如果它失败了，这将极大地限制主要研究人员的研究，他们完全依赖于它来追踪生物系统中的铜和铁；在铜和铁结合蛋白及其受体的纯化和/或结合能力、动力学和功能的研究中追踪它们；通过亲和标记鉴定特定酶的底物和辅因子的结合部位。所使用的同位素(67Cu、64Cu、59Fe、125I)都是伽马发射体，经常需要双重标记。

The objective of this proposal is to acquire a high quality, automatic FPLC system and a new gamma counter, to purify and track proteins/peptides (particularly radioactive proteins/peptides) and their metals or prosthetic groups. The FPLC system is needed because the laboratories of the primary investigators currently have only very limited access to such systems, although in each, purification of proteins/peptides (for determination of their structure, function and regulation) is a major activity. A new gamma counter is needed because the existing one is twelve years old and threatening to become unreliable. If it should fail, this would greatly limit the research of the primary investigators, who are utterly dependent upon it for tracing copper and iron in biological systems; tracking copper and iron binding proteins and their receptors during their purification and/or in studies of their binding capacities, kinetics and functions; identifying the binding sites of substrates and cofactors of specific enzymes via affinity labeling. The isotopes used (67Cu,64Cu,59Fe, 125I) are all gamma emitters, and double labeling is often required.