Cloning the Shootless Locus of Arabidopsis Thaliana

克隆拟南芥无芽基因座

• 批准号: 9222013
• 负责人: M. Kathryn Barton
• 金额: $ 3.5万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1993-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9222013&HistoricalAwards=false
• 关键词: Cloning; Shootless; Locus; Arabidopsis; Thaliana

Plant meristems are located at the tips of growing roots and shoots. They are comprised of densely cytoplasmic, undifferentiated, dividing cells. These cells have properties characteristic of "stem cells" in that their descendants give rise not only to more undifferentiated, meristematic cells but also to the many different cell types that make up the plant body. Thus, understanding how shoot apical meristems work is not only critical to understanding the development of plants but will also shed light on the more general problem of how undifferentiated "stem cell" populations of cells generate complex structures comprised of many different cell types. This new investigator proposes to identify, isolate and characterize a clone the wild-type DNA encoding the SHOOTLESS locus of Arabidopsis. Possible practical applications of this research include the ability to more precisely control shoot regeneration. The data thus far suggest that the SHOOTLESS locus is necessary for the formation of a shoot apical meristem. It is possible that in addition to being necessary for shoot meristem formation, the SHOOTLESS product is also sufficient for shoot meristem formation. If this is true, an increase in the expression of the SHOOTLESS product would increase the likelihood that a given tissue would regenerate shoots. Learning how to control such a gene would result in an increased ability to regenerate shoots from tissues or species that are otherwise recalcitrant to shoot regeneration protocols. This would accelerate the creation of new varieties of commercially important plant species.//

植物分生组织位于生长的根的顶端， 芽 它们由密集的细胞质， 未分化的分裂细胞 这些细胞具有 “干细胞”的特征在于它们的后代产生 不仅是未分化的分生组织细胞， 构成植物体的多种不同细胞类型。 因此，在本发明中， 了解茎尖分生组织的工作方式不仅对 了解植物的发展， 关于未分化的“干细胞” 细胞群产生复杂的结构， 不同的细胞类型 这位新的研究人员建议识别，分离和 表征编码以下的野生型DNA的克隆： 拟南芥的无芽基因座。 可能的实际应用 包括能够更精确地控制 芽再生 目前的数据表明， 位点是茎尖分生组织形成所必需的。 有可能除了拍摄所必需的 分生组织的形成，SHOOTLESS产品也足以 茎分生组织形成。 如果这是真的， SHOOTLESS产物的表达将增加 一个特定的组织可以再生出芽。 学习如何 控制这样的基因将导致增加的能力， 从其他组织或物种中再生出枝条 芽再生方案的可行性。 这将 加快商业上重要的新品种的创造 植物种类。