Cryopreservation of the UTEX Algal Culture Collection

UTEX 藻类培养物保藏的冷冻保存

• 批准号: 9224425
• 负责人: Jerry Brand
• 金额: $ 11万
• 依托单位: University of Texas at Austin
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1996-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9224425&HistoricalAwards=false
• 关键词: Cryopreservation; UTEX; Algal; Culture; Collection

9224425 Brand This award supports a feasibility study to determine if a reliable and practical method to cryopreserve algal strains can be developed. Specifically, Dr. Brand, in collaboration with Dr. Diller and Dr. Starr will develop means to cryopreserve the strains of algae in the University of Texas Culture Collection of Algae. Cryopreservation, if successful, will (1) stabilize and protect the algal resources, (2) diminish the day-to-day cost of maintaining the collection, and (3) make storage and shipment less expensive and more efficient. Prokaryotic algae (cyanobacteria) in general are amenable to storage under conditions currently used to cryopreserve other bacteria. Recent advances in techniques for cryopreservation of other organisms indicate that many of the eukaryotic algal strains will be amenable to cryopreservation without a major experimental effort. Protocols will be developed which permit cryopreserved cultures to be thawed and grown in small batches, thus making these generic resources available and useful for collections anywhere in the world which maintain algal strains for research, teaching and/or technology development. ***

9224425品牌 该奖项支持一项可行性研究，以确定是否可以开发一种可靠和实用的方法来冷冻保存藻类菌株。 具体来说，Brand博士与Diller博士和Starr博士合作，将开发在德克萨斯大学藻类培养物保藏中心冷冻保存藻类菌株的方法。 冷冻保存如果成功，将（1）稳定和保护藻类资源，（2）减少日常维护收集的成本，（3）使储存和运输更便宜，更有效。 原生藻类（蓝细菌）通常适合于在目前用于冷冻保存其他细菌的条件下储存。 最近在其他生物体的冷冻保存技术方面的进展表明，许多真核藻类菌株将适合于冷冻保存，而无需进行重大的实验努力。 将制定协议，允许冷冻保存的培养物解冻和小批量生长，从而使这些通用资源可用于世界任何地方的收藏，以保持藻类菌株用于研究，教学和/或技术开发。 ***