Cryopreservation of the UTEX Culture Collection of Algae

UTEX 藻类培养物保藏的低温保存

• 批准号: 9808891
• 负责人: Jerry Brand
• 金额: $ 14.73万
• 依托单位: University of Texas at Austin
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-10-01 至 2001-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9808891&HistoricalAwards=false
• 关键词: Cryopreservation; UTEX; Culture; Collection; Algae

9808891BrandThis award renews support of efforts to define effective methods for cryopreservation of a large variety of single-celled algae (microalgae) including over 2000 strains found in the UTEX collection of freshwater algae directed by the principal investigator of this award. Such microalgae are used for basic research in genetics, ecology and physiology, and for varied commercial purposes. Development of reliable methods for their cryopreservation will eliminate the need for maintenance of strains through serial culture techniques. Serial culture techniques are costly and, as importantly, permit the genetic makeup of the strains to change over time, thereby limiting their usefulness for some types of research. Thus the development of reliable cryopreservation methods is of scientific as well as economic importance to the researchers and others who use microalgae. Progress to date suggests that well over 50% of the strains in the UTEX collection can be cryopreserved. Work supported through this work will allow completion of the testing of the collection and additional efforts aimed at development of new methods for dealing with those strains so far refractory to existing cryopreservation methodology.

该奖项再次支持了为确定多种单细胞藻类（微藻类）的有效低温保存方法所做的努力，其中包括由该奖项首席研究员指导的UTEX淡水藻类收集中发现的2000多种菌株。这些微藻被用于遗传学、生态学和生理学的基础研究，以及各种商业目的。可靠的冷冻保存方法的发展将消除通过连续培养技术维持菌株的需要。连续培养技术是昂贵的，而且同样重要的是，它允许菌株的基因组成随着时间的推移而改变，从而限制了它们对某些类型研究的有用性。因此，开发可靠的低温保存方法对研究人员和其他使用微藻的人具有科学和经济意义。迄今为止的进展表明，UTEX收集的50%以上的菌株可以冷冻保存。通过这项工作支持的工作将允许完成收集的测试，并进一步努力开发新的方法来处理那些迄今为止对现有冷冻保存方法难以处理的菌株。